Mass spectrometry method using chromatography-mass spectrometry device

A technology of mass spectrometry and chromatography, applied in the field of mass spectrometry for the determination of proteins, peptides and nucleic acids, can solve complex and uncomfortable problems

Pending Publication Date: 2022-01-07
SHIN NIPPON BIOMEDICAL LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The device is complex and unsuitable for mass spectrometry analysis of proteins, peptides and nucleic acids

Method used

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  • Mass spectrometry method using chromatography-mass spectrometry device
  • Mass spectrometry method using chromatography-mass spectrometry device
  • Mass spectrometry method using chromatography-mass spectrometry device

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Experimental program
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Embodiment 1

[0061] Experimental Materials

[0062] The LNA (14mer oligonucleotide, molecular weight 4572.6) to be measured was obtained from the National Center for Circulatory Diseases (NCVC). 1,1,1,3,3,3-hexafluoro-2-propanol (for HFIPA, for high performance liquid chromatography) was purchased from Kanto Chemical Co., Ltd. N,N was purchased from Fujifilm Wako Pure Chemical Industries, Ltd. - Diisopropylethylamine (DIPEA, Hünig base, reagent special grade) and triethylamine (TEA, reagent special grade). As other pharmaceuticals used, special grade reagents were selected and purchased from Fujifilm Wako Pure Chemical Industries. Solid-phase extraction was performed using an OASIS HLB 96-well plate (30 mg, manufactured by Waters Corporation). As the HPLC column, a CADENZA CD-C18 3 μm HPLC column with an inner diameter of 2.1 mm and a length of 50 mm (manufactured by IMTAKT) was used. To prevent evaporation of the mobile phase, A-Line safety caps manufactured by Agilent were used.

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Abstract

To provide a mass spectrometry method that uses a chromatography-mass spectrometry device, wherein mass spectrometry can be performed with a convenient method at low cost. A mass spectrometry method for a target object, using a chromatography-mass spectrometry device that includes, in order, a chromatograph, an ionization unit, a first mass separation unit, a collision cell, a second mass separation unit, and an ion detection unit, wherein an ion pair agent and Huenig's base are added to a mobile phase of the chromatograph, and the absolute value of an applied voltage of the collision cell is 20V to 250V.

Description

technical field [0001] The invention relates to a mass spectrometry method using chromatography-mass spectrometer devace. In particular, the present invention relates to a mass spectrometry method capable of appropriately measuring proteins, peptides, and nucleic acids. Background technique [0002] A chromatographic mass spectrometry device is described in International Publication No. 2016-125271. The device suffers from complexity and is not suitable for mass spectrometric analysis of proteins, peptides and nucleic acids. [0003] [Prior Art Document] [0004] [Patent Document] [0005] Patent Document 1: International Publication No. 2016-125271 Contents of the invention [0006] [Technical problem to be solved by the invention] [0007] An object of one invention described in this specification is to provide a mass spectrometry method using a chromatographic mass spectrometer that can perform mass spectrometry by a low-cost and simple method. In particular, in o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/72G01N27/62H01J49/00
CPCG01N30/02G01N30/34G01N30/7233G01N27/62H01J49/004H01J49/005H01J49/0027G01N30/88G01N30/86G01N30/72G01N2030/8831G01N2030/027
Inventor 千田直人坟崎靖子
Owner SHIN NIPPON BIOMEDICAL LAB
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