Urine sample treatment and detection method

The technology of a urine sample and a detection method is applied in the field of urine sample processing and detection, which can solve problems such as large errors, and achieve the effects of simple detection, low cost and wide application range.

Pending Publication Date: 2022-01-11
邱永丰
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  • Description
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Problems solved by technology

[0004] In order to solve the defect that the stone detection method in the prior art has large errors, the present invention provides a urine sample processing and detection method

Method used

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  • Urine sample treatment and detection method
  • Urine sample treatment and detection method

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Experimental program
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Embodiment

[0026] A method for processing and detecting a urine sample, comprising the following steps:

[0027] Step 1, collect samples:

[0028] Collect the urine of the person to be tested, collect the urine with a collection tube with a cover within 1 hour after the fresh urine is discharged, and place it on a multifunctional oscillator at 20rpm for 1 minute, draw 5ml of urine with a 5ml syringe and put it into a small sterile test tube as a pre-processing urine sample;

[0029] Step 2, process the sample:

[0030] After drawing 5ml of urine with a 5ml syringe, connect the microfiltration membrane processor (ie 0.22μm water needle filter) to filter the urine into a small test tube as the processed urine sample;

[0031] Step 3, test samples and numerical calculations:

[0032] The urine sample before treatment and the urine sample after treatment were respectively taken for microplate detection, and the wavelength (λ) 405nm, 450nm, 490nm was used as the detection condition to obta...

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Abstract

The invention discloses a urine sample treatment and detection method which is characterized by comprising the following steps; 1, collecting and detecting a sample: collecting urine of a to-be-detected person, and detecting the absorbance value OD0 of the urine; 2, treating and detecting the sample: treating the urine sample to be detected by using a microfiltration membrane, and detecting the absorbance value OD1 of the urine again; 3, numerical calculation and analysis: obtaining a target numerical value by using the absorbance value OD0 and the absorbance value OD1 to analyze the urinary calculus lithiasis risk tendency of the urine sample, wherein if the target value is smaller than 1, the result is positive, the urine sample has the lithiasis risk tendency, and the smaller the target value is, the larger the lithiasis risk tendency is possible, and if the target value is not less than 1, the result is negative, and the urine sample has no lithogenic risk tendency. The method has the advantages of simplicity and convenience in detection, low cost and wide application range, and opens up a new way for research on urinary calculus.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a method for processing and detecting urine samples. Background technique [0002] Urinary calculus is one of the most common urological diseases in my country, and southern China is one of the three high-incidence areas in the world. About 50% of patients will relapse within 10 years. There is no simple, convenient and effective urinary system for urinary calculi. The urine test method is used to analyze the risk tendency of urine stone formation, and even evaluate the curative effect of stone treatment drugs. [0003] At present, when urine stones are detected, the determination method of oxalic acid and citric acid-ion chromatography is mostly used, but it has inherent defects. In principle, ion chromatography is a 20-year-old technology that detects the electrical signal generated by the ion exchange in the liquid through a detector to achieve the determination of the concentra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/01
CPCG01N21/31G01N21/01G01N2021/0112
Inventor 邱永丰
Owner 邱永丰
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