Flow cytometry multispectral analyzer and use method thereof

A technology of flow cytometry and spectrum analysis, applied in the direction of material analysis, material excitation analysis, material analysis by electromagnetic means, etc., can solve the problem of few analysis methods, achieve the effect of ensuring the detection throughput and realizing the detection of a large number of cells

Pending Publication Date: 2022-02-08
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the combination of protein and metabolomics is still in its infancy, and there are still relatively few related analysis methods

Method used

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  • Flow cytometry multispectral analyzer and use method thereof
  • Flow cytometry multispectral analyzer and use method thereof
  • Flow cytometry multispectral analyzer and use method thereof

Examples

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Embodiment 1

[0033] In this example, if figure 1 As shown, a flow cytometry multispectral analyzer is provided, which includes a sampling module, a spectral detection module 1, an ionization chamber 2, a mass spectrometric detection module and a data processing module. After the sample injection module transmits the output continuous liquid flow to the spectrum detection module 1 for processing, the continuous liquid flow is transmitted to the ionization chamber 2, and the electrical signal obtained after being processed by the spectrum detection module 1 is transmitted to the data processing module; the ionization chamber 2 The processed ions enter the mass spectrometry detection module for processing, and the processed results are transmitted to the data processing module; the data processing module integrates all the received data.

[0034] Among them, the sampling module is used for the introduction of cell suspension and the linear focusing of cells; the sampling module includes a sam...

Embodiment 2

[0050] In this embodiment, based on the flow cytometry multispectral analyzer in Example 1, a method for using the flow cytometry multispectral analyzer is provided. In this embodiment, human tumor cells HeLa and MCF7 are used as research objects , the usage method includes the following steps:

[0051] Step 1. Add the cell suspension to be tested into the sampling pool 4, turn on the sampling pump 3 to stably introduce the cell suspension into the focusing flow path 5, and after focusing, the cells are arranged in a single row in the continuous liquid flow and enter the optical detection module 4;

[0052] In this embodiment, human tumor cells HeLa and MCF7 were used as research objects, and spectral analysis was used to distinguish the types of tumor cells, and then mass spectrometry was used to obtain the differences in metabolite levels of different types of immune cells.

[0053] Fluorescence staining is performed on the cell samples to prepare a pre-separated tumor cell...

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Abstract

The invention relates to a flow cytometry multispectral analyzer and a use method thereof. The flow cytometry multispectral analyzer comprises a sample introduction module, a spectrum detection module, an ionization chamber, a mass spectrum detection module and a data processing module; the sample introduction module transmits output continuous liquid flow into the spectrum detection module for processing, then the continuous liquid flow is transmitted into the ionization chamber, and an electric signal obtained after the continuous liquid flow is processed by the spectrum detection module is transmitted to the data processing module; ions obtained after treatment of the ionization chamber enter the mass spectrum detection module to be processed, and the processed result is transmitted to the data processing module; and the data processing module is used for integrating all the received data. The multispectral high-throughput detection of multidimensional information such as cell protein and metabolite is realized by combining spectral analysis and mass spectrometry. The invention can be widely applied to the technical field of cell analysis.

Description

technical field [0001] The invention relates to the technical field of cell analysis, in particular to a flow cytometry multispectral analyzer and a method for using the same. Background technique [0002] Cell-by-cell analysis helps uncover differences that are masked by population cell averaging. With the continuous development of analysis technology, many analysis technologies at the single-cell level have emerged, which can perform high-throughput analysis of important biomolecules in a single cell. Single-cell analysis technology initially focused on the development of molecular information that can accurately determine a single type of cell, such as using technologies such as single-cell DNA sequencing to study cell genomes, using technologies such as single-cell RNA sequencing to study cell transcriptomes, and using technologies such as flow cytometry Study protein expression in cells, study cell metabolome with techniques such as mass spectrometry. [0003] In rece...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N27/626
CPCG01N21/6402G01N27/626
Inventor 张新荣潘星宇姚欢张四纯
Owner TSINGHUA UNIV
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