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Method for acquiring Y chromosome sequence of pseudobagrus ussuriensis

A technology of Y chromosome and acquisition method, which is applied in the field of acquisition of the Y chromosome sequence of the Ussuri quail, can solve the problems of limited knowledge and inability to meet all-male breeding, etc., so as to promote the breeding process, shorten the required time, and improve work efficiency. Effect

Active Publication Date: 2022-02-18
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

However, the current understanding of the sex-determining mechanism of P. ussurii is very limited and cannot meet the needs of all-male breeding.

Method used

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Embodiment 1

[0025] A method for obtaining the Y chromosome sequence of Pseudomonas Ussuri, comprising the steps of:

[0026] Step 1: Build an interspecies hybrid population: in May 2021, select 6 female Pseudomonas spp. with swollen, soft and elastic abdomens, visible ovary outlines on the abdomen, and no injuries, with an average weight of 95 grams; select reproductive tips There are 3 long, reddish-ended, strong and healthy male Ussuri catfish with an average weight of 268 grams; after the parents are selected, they are kept in a circular spawning pond with a diameter of 1.7 meters for 3 days. At 7:00 on May 20, 2021, inject the first injection of oxytocic agent, and inject 5 micrograms / kg of LRH-A to each female Oleopsis 2 , the male Ussuri bream was not injected. At 19:00 on May 20th, the second dose of oxytocic agent was injected, and each female Oleopsis chinensis was administered according to LRH-A 2 10 micrograms + HCG 1500 units + DOM 5 mg / kg injection, each male Ussuri fish a...

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Abstract

The invention relates to the technical field of neutral chromosome analysis in aquaculture, and discloses a method for acquiring a Y chromosome sequence of pseudobagrus ussuriensis, which comprises the following steps: step 1, constructing an interspecific hybrid population which is obtained from male pseudobagrus ussuriensis and female Pseudobagrus tenuis through artificial insemination; step 2, identifying male hybrid individuals, wherein the male hybrid individuals in the step 2 are identified through a gonad characteristic observation method and a molecular marker method; step 3, sequencing and assembling the third-generation genomes of the male hybrid individuals, wherein the sequencing and assembling of the third-generation genomes in the step 3 are implemented by adopting HiFi and Hi-C technologies; and step 4, conducting comparing with a female genome chromosome to obtain a Y chromosome sequence. According to the method for obtaining the Y chromosome sequence of the pseudobagrus ussuriensis, the time required for obtaining the Y chromosome sequence under the condition of the prior art can be greatly shortened, the labor intensity is effectively reduced, the working efficiency is improved, and the sex control breeding process of the pseudobagrus ussuriensis is powerfully promoted.

Description

technical field [0001] The invention relates to the technical field of neutral chromosome analysis in aquaculture, in particular to a method for obtaining the Y chromosome sequence of the Ussuri fish. Background technique [0002] Sexual dimorphism is common in fish. In some fish, males grow faster than females, while in some fishes, females grow faster than males. In fish breeding, if the sex growth difference can be fully utilized to breed all-male or all-female strains, it will greatly promote the improvement of the corresponding fish breeding production. In order to achieve the goal of sex-controlled breeding, it is first necessary to understand the sex-determining mechanism of the target fish, and obtaining sex chromosome sequences is an important breakthrough in revealing its sex-determining mechanism. [0003] At present, for XX-XY sex-determining fish, the main methods to obtain the Y chromosome sequence are: 1. Use estrogen to treat the gonad undifferentiated larva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/6806C12Q1/6869
CPCC12Q1/6888C12Q1/6879C12Q1/6806C12Q1/6869C12Q2535/122Y02A40/81
Inventor 朱传坤潘正军刘海洋王辉常国亮丁怀宇聂孝燕程磊孙艳红余祥胜
Owner HUAIYIN TEACHERS COLLEGE
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