Incised notopterygium seed tissue culture breeding method

A seed and notopterygium technology, applied in the field of plant tissue culture, can solve the problems of low survival rate, hidden dangers of notopterygium production expansion, only focus on cultivation cycle, reproduction coefficient, etc., and achieve good growth, good induction effect, and good growth condition

Active Publication Date: 2022-03-15
甘孜藏族自治州农业科学研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plant seeds are cultivated using tissue culture medium. Due to problems such as root induction rate, seedling growth rate, and growth in the tissue culture stage, the survival rate after transplanting is often low, and ultimately it is impossible to achieve a large number of plants. The notopterygium seed group of this patent The cultivation and breeding technology only pays attention to the cultivation cycle and reproduction coefficient. Since the influence of other factors on tissue culture seedlings is not considered more comprehensively, the expansion of notopterygium in the later stage of transplanting faces a huge risk, which brings hidden dangers to the expanded production of notopterygium

Method used

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  • Incised notopterygium seed tissue culture breeding method
  • Incised notopterygium seed tissue culture breeding method
  • Incised notopterygium seed tissue culture breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Aseptic Germination and Rapid Propagation of Notopterygium Seeds

[0051] Step 1, pre-treatment of notopterygium seeds: the notopterygium seeds are layered in wet sand at 25°C for 3 months, and then at 4°C for 3 months, then the seeds are taken out, rinsed with distilled water, and placed on the Germination experiments were carried out in a culture dish with filter paper at a temperature of 15°C, a humidity of 75%, a light intensity of 2200Lux, and a photoperiod of 12h (light) / 12h (dark). After 1 month, ungerminated seeds were collected, mixed evenly, placed on moistened sterile filter paper, and stored in a 4°C refrigerator.

[0052] Step 2, selection and treatment of notopterygium seed explants: select healthy seeds, peel off the outer testa of the seeds, soak in soapy water for 1 minute, rinse under running water for 30 minutes, and set aside;

[0053] Step 3, disinfection of notopterygium seeds: soak and disinfect the notopterygium seeds treated in step 2...

Embodiment 2

[0057] Example 2 Aseptic Germination and Rapid Propagation of Notopterygium Seeds

[0058] Step 1, pre-treatment of notopterygium seeds: the notopterygium seeds are layered in wet sand at 25°C for 3 months, and then at 4°C for 3 months, then the seeds are taken out, rinsed with distilled water, and placed on the Germination experiments were carried out in a culture dish with filter paper at a temperature of 15°C, a humidity of 75%, a light intensity of 2200Lux, and a photoperiod of 12h (light) / 12h (dark). After 1 month, ungerminated seeds were collected, mixed evenly, placed on moistened sterile filter paper, and stored in a 4°C refrigerator.

[0059] Step 2, selection and treatment of notopterygium seed explants: select healthy seeds, peel off the outer testa of the seeds, soak in soapy water for 1 minute, rinse under running water for 30 minutes, and set aside;

[0060] Step 3, disinfection of notopterygium seeds: soak and disinfect the notopterygium seeds treated in step 2...

Embodiment 3

[0064] Example 3 Aseptic Germination and Rapid Propagation of Notopterygium Seeds

[0065] Step 1, pre-treatment of notopterygium seeds: the notopterygium seeds are layered in wet sand at 25°C for 3 months, and then at 4°C for 3 months, then the seeds are taken out, rinsed with distilled water, and placed on the Germination experiments were carried out in a culture dish with filter paper at a temperature of 15°C, a humidity of 75%, a light intensity of 2200Lux, and a photoperiod of 12h (light) / 12h (dark). After 1 month, ungerminated seeds were collected, mixed evenly, placed on moistened sterile filter paper, and stored in a 4°C refrigerator.

[0066] Step 2, selection and treatment of notopterygium seed explants: select healthy seeds, peel off the outer testa of the seeds, soak in soapy water for 1 minute, rinse under running water for 30 minutes, and set aside;

[0067] Step 3, disinfection of notopterygium seeds: soak and disinfect the notopterygium seeds treated in step 2...

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Abstract

The invention discloses a culture medium for notopterygium seed tissue culture breeding and a notopterygium seed tissue culture breeding method. Experiments prove that the survival rate of tissue culture seedlings obtained by the notopterygium seed tissue culture breeding method after transplanting reaches 90% or above, a solid foundation is laid for mass propagation of notopterygium, and the notopterygium seed tissue culture breeding method has popularization and application values.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture and breeding of notopterygium seeds. Background technique [0002] Notopterygium incisum Ting ex H.T.Chang is a perennial herb of the genus Notopterygium in the Umbelliferae family (Umbelliferae), endemic to my country. According to the Pharmacopoeia, the medicinal parts of notopterygium are dry rhizomes and roots. The main chemical components are notopterygium alcohol, isoimperatorin and other compounds. Strong, rheumatic arthralgia, shoulder and back pain. Notopterygium is the prescription medicinal material of many famous patent medicines, among which the classic medicine for relieving wind and cold is Jiuwei Qianghuo Pill, and the classic medicine for expelling wind and dampness is Qianghuo Shengshi Decoction. Due to the good medicinal effects of notopterygium, it is loved by people and has a very good market value. [0003] Notopterygium gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/40
Inventor 丰先红张利苏娜杨瑞武姜媛媛倪甦廖进秋王龙雷高赵艳妮
Owner 甘孜藏族自治州农业科学研究所
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