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Tissue culture and rapid propagation method for producing spiranthes sinensis seedlings by using leaves

A technique for tissue culture, rapid propagation and Panlong ginseng, which is applied in the directions of horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as the propagation of Panlong ginseng seedlings, reduce seedling loss rate, save production costs, Conducive to domestication and survival

Active Publication Date: 2022-03-18
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a method for tissue culture and rapid propagation of Panax ginseng seedlings produced by leaves, so as to solve the problem of Panax ginseng seedling propagation

Method used

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  • Tissue culture and rapid propagation method for producing spiranthes sinensis seedlings by using leaves
  • Tissue culture and rapid propagation method for producing spiranthes sinensis seedlings by using leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Selection of explants: Transplant healthy and pest-free Panlong ginseng into indoor flowerpots, spray 70% carbendazim 500 times liquid every 2 to 3 days, spray 3 times continuously, and then take Panlong ginseng heart leaves.

[0024] 2. Cleaning of explants: Rinse Panlong ginseng heart leaves with tap water for 80 minutes, place in soapy water, rinse for 10 minutes, rinse 4 times with tap water, then drop 3 drops of Tween-80, shake for 8 minutes, Finally, rinse with tap water for 80 minutes.

[0025] 3. Disinfection of explants: transfer the cleaned explants to an ultra-clean workbench, disinfect them with 75% alcohol for 20 seconds, rinse them with sterile water twice, and disinfect them with 8% hydrogen peroxide for 40 minutes before using Rinse with sterile water 3 times, and finally sterilize with 0.05% mercury liter solution for 9 minutes, then rinse with sterile water 8 times. The 7-day disinfection contamination rate was 17.6%, and the 20-day leaf mortality...

Embodiment 2

[0031] 1. Selection of explants: Transplant healthy and pest-free Panlong ginseng into indoor flowerpots, spray 70% carbendazim 500 times liquid every 2 to 3 days, spray 3 times continuously, and then take Panlong ginseng heart leaves.

[0032] 2. Cleaning of explants: Rinse Panlong ginseng heart leaves with tap water for 80 minutes, place in soapy water, rinse for 15 minutes, rinse 3 times with tap water, then drop 3 drops of Tween-80, shake for 10 minutes, Finally, rinse with tap water for 80 minutes.

[0033] 3. Disinfection of explants: transfer the cleaned explants to the ultra-clean workbench, disinfect them with 75% alcohol for 15 seconds, rinse them with sterile water twice, and disinfect them with 8% hydrogen peroxide for 35 minutes before using them. Rinse with sterile water 2 to 3 times, and finally disinfect with 0.05% mercury liter solution for 8 minutes, then rinse with sterile water 8 times. The 7-day disinfection contamination rate was 18.2%, and the 20-day l...

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Abstract

The invention relates to the field of medicinal plant tissue culture, and particularly discloses a tissue culture and rapid propagation method for producing spiranthes sinensis seedlings by using leaves, which comprises the following steps: selecting tender heart leaves of spiranthes sinensis as explants, cleaning and disinfecting; the method comprises the following steps: inducing germination: inoculating a disinfected explant to improved MS, 0.9 to 1.2 mg / L of 2, 4-D, 1.2 to 1.6 mg / L of TDZ and 0.4 g / L of activated carbon; proliferation from sprouting: cutting the induced cluster buds into leaves with the length of 1.0 cm, and respectively inoculating the leaves and non-leaf cluster single buds into a proliferation culture medium combined by improved MS, 0.6-0.8 mg / L of NAA, 2.0-2.4 mg / L of TDZ and 80g / L of coconut juice; rooting of cluster buds: cutting the cluster buds obtained by proliferation into single buds, and inoculating the single buds into a rooting culture medium composed of 1 / 3 improved MS, 1.5-2.0 mg / L IBA, 0.4-0.7 mg / L paclobutrazol, 50g / L coconut juice and 0.8 g / L activated carbon; according to the method disclosed by the invention, the multiple shoots are directly induced by using the leaves, so that the leaves are not discarded during multiplication inoculation in the actual production process and become materials capable of continuously producing the multiple shoots, and the multiplication coefficient is greatly improved.

Description

technical field [0001] The invention relates to the field of medicinal plant tissue culture, in particular to a method for tissue culture and rapid propagation of Panax ginseng seedlings produced by using leaves. Background technique [0002] Spiranthes sinensis (Per.) Ames is a perennial herbaceous plant of the family Orchidaceae. It is mainly produced in Yunnan, Sichuan, Zhejiang, Hunan, Jiangxi, Henan and other places, and it is also distributed in other provinces, regions and cities in China. Panlong ginseng mainly grows in hillside forests, shrubs, grasslands and river marsh meadows at an altitude of 200-3400 meters. Panlong ginseng was first recorded in "Southern Yunnan Materia Medica". The root or whole herb is used as medicine. It has the effects of nourishing qi and nourishing yin, clearing away heat and detoxifying. , nocturnal emission, stranguria leucorrhea, sore throat, snake bites, burns, bloated sores. Modern medical research Panlong ginseng contains dihydr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02A40/22
Inventor 张金渝杨天梅许宗亮左应梅杨绍兵朱新焰曾祥飞赵露琴
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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