Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof

A coronavirus and sars-cov-2 technology, applied in the direction of antiviral agents, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as toxic side effects and patient injury, achieve less toxic side effects and lower drug prices , the effect of inhibiting infection

Pending Publication Date: 2022-03-18
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above drugs have toxic side effects and may cause unnecessary damage to the patient itself, so finding a less toxic anti-SARS-CoV-2 drug has become an urgently needed solution for the treatment of COVID-19

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof
  • Medicine for resisting novel coronavirus SARS-CoV-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] African green monkey kidney cell Vero was purchased from ATCC; SARS-CoV-2 virus was isolated from the sample; one-step absolute quantitative PCR enzyme Fast Virus 1-Step Master Mix (4444434), Trizol LS was purchased from Thermo; nucleic acid extraction kit Direct- zol TMRNA MiniPrep (R2052) was purchased from Zymo Research; CCK8 kit (40203) was purchased from Shanghai Yisheng Biotechnology Co., Ltd.

[0025] 1. RNA extraction from the supernatant

[0026] Follow the instructions of the nucleic acid extraction kit, as follows:

[0027] 1) Add an equal volume of absolute ethanol to the sample added with Trizol LS, mix well and transfer the liquid to Zymo-Spin TM On the IICR column, the column is placed on the collection tube and centrifuged at 10000g for 1min

[0028] 2) Put the Zymo-Spin TM IICR column transferred to a new collection tube

[0029] 3) On Zymo-Spin TM Add 400 μl of Direct-zol to IICR column TM RNA PreWash, centrifuge at 10000g for 1min, discard ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a series of anti-novel coronavirus SARS-CoV-2 drugs and application thereof, in vitro cell test results show that the compound can significantly inhibit the infection of SARS-CoV-2 on normal cells, has an inhibition effect on novel coronavirus SARS-CoV-2 at the cellular level, can significantly reduce the virus titer of the virus in the cells, and can be used for preparing anti-novel coronavirus SARS-CoV-2 drugs. The proliferation of the compound in cells is inhibited, and the compound has dose dependence. Therefore, the compound can be used as a novel coronavirus SARS-CoV-2 inhibitor, and has the potential of treating COVID-19 pneumonia caused by human infection or other diseases caused by animal infection.

Description

technical field [0001] The present invention belongs to the field of virology and medicinal chemistry, and in particular relates to a compound that inhibits the replication of the novel coronavirus SARS-CoV-2 virus, the mechanism by which the compound inhibits the replication of the SARS-CoV-2 virus, and the use of the compound in preventing and treating SARS-CoV-2 -Related diseases caused by CoV-2 virus, especially the use on diseases related to inflammation and autoimmunity. Background technique [0002] The novel coronavirus SARS-CoV-2, the Middle East respiratory syndrome coronavirus MERS-CoV, and the severe acute respiratory syndrome coronavirus SARS-CoV belong to the family Coronaviridae. Strand RNA virus, whose genome length is about 26000-32000bp, is the largest RNA virus known so far. [0003] There is an urgent need for effective antiviral drugs to combat the infection of SARS-CoV-2, which can not only reduce the disease burden of patients, but also reduce the ris...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K45/06A61P31/14A61P11/00
CPCA61K45/06A61P31/14A61K45/00A61P11/00
Inventor 王健伟雷晓波肖霞任丽丽王聪慧
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap