Gene ZmNR2 for regulating and controlling corn stalk puncture strength and application of molecular marker of gene ZmNR2
A technology of puncture strength and molecular markers, which can be used in applications, genetic engineering, genomics, etc., and can solve problems such as complex genetic basis
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[0031] 1. Materials and methods
[0032] 1.1 Plant material and phenotypes
[0033] The associated group includes 345 maize inbred lines from different regions in China, and the 345 inbred lines are numbered (A001-A478). These inbred lines were subjected to three field trials in Sanya from 2015 to 2017. Each field experiment adopts a completely randomized block design, one row block, two repetitions. Each row consisted of 13 plants, 3.5 meters long and 0.5 meters wide. At the flowering stage, 9 plants per line were randomly selected from each replicate for phenotypic identification. The mean values of 7 traits including SD, SL, PH, EH, SBS, SCS and RPR were calculated. Except for PH and EH, the rest of the traits were measured at 2 weeks after flowering by analyzing the middle part of the flat edge of the third internode. Specifically, SBS, SCS, and RPR were measured using different pressure transducers of the YYD-1 instrument (Zhejiang Top Cloud Agricultural Technology...
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