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Gene ZmNR2 for regulating and controlling corn stalk puncture strength and application of molecular marker of gene ZmNR2

A technology of puncture strength and molecular markers, which can be used in applications, genetic engineering, genomics, etc., and can solve problems such as complex genetic basis

Pending Publication Date: 2022-03-18
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some progress has been made in the QTL mapping of maize lodging resistance-related traits, the genetic basis of maize lodging is affected by multiple traits, and there are large differences in different genetic backgrounds.

Method used

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  • Gene ZmNR2 for regulating and controlling corn stalk puncture strength and application of molecular marker of gene ZmNR2
  • Gene ZmNR2 for regulating and controlling corn stalk puncture strength and application of molecular marker of gene ZmNR2
  • Gene ZmNR2 for regulating and controlling corn stalk puncture strength and application of molecular marker of gene ZmNR2

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Embodiment 1

[0031] 1. Materials and methods

[0032] 1.1 Plant material and phenotypes

[0033] The associated group includes 345 maize inbred lines from different regions in China, and the 345 inbred lines are numbered (A001-A478). These inbred lines were subjected to three field trials in Sanya from 2015 to 2017. Each field experiment adopts a completely randomized block design, one row block, two repetitions. Each row consisted of 13 plants, 3.5 meters long and 0.5 meters wide. At the flowering stage, 9 plants per line were randomly selected from each replicate for phenotypic identification. The mean values ​​of 7 traits including SD, SL, PH, EH, SBS, SCS and RPR were calculated. Except for PH and EH, the rest of the traits were measured at 2 weeks after flowering by analyzing the middle part of the flat edge of the third internode. Specifically, SBS, SCS, and RPR were measured using different pressure transducers of the YYD-1 instrument (Zhejiang Top Cloud Agricultural Technology...

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Abstract

The invention discloses application of a gene ZmNR2 for regulating and controlling corn stalk puncture strength and a molecular marker of the gene ZmNR2, and belongs to the field of molecular biology, the corn ZmNR2 gene has the effect of regulating and controlling the corn stalk puncture strength, the nucleotide sequence of the corn ZmNR2 gene is as shown in SEQ ID No.1, at the upstream 1.6 kb position of S5210786313, the SNP molecular marker of the corn ZmNR2 gene is located at the 623bp position of the corn ZmNR2 gene, and the basic group at the position is C or A. The gene ZmNR2 related to the stalk puncture strength character is screened in a corn genome through a genome-wide association analysis (GWAS) technical method, the gene plays a very key role in regulating and controlling the corn stalk strength, and the discovery may contribute to clarification of the genetic basis of the stalk strength; in addition, the identified candidate gene and variation have potential breeding value, and can be used for molecular marker-assisted selective breeding to improve lodging resistance of corn.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a gene ZmNR2 for regulating the puncture strength of corn stalks and the application of molecular markers thereof. Background technique [0002] Maize (Zea mays L) is an important food, economic and feed crop in my country, and occupies a pivotal position in the entire national economy. With the increase of corn planting density and the frequent occurrence of natural disasters, corn lodging tends to intensify. For every 1% increase in corn lodging rate, about 108kg / hm2 of yield will be reduced, and the annual loss of corn yield due to lodging in the world is 5 %~20%. Lodging not only seriously affects the yield and quality of corn, but also brings obstacles to corn mechanical harvesting. It is one of the important limiting factors for high, stable and high-quality corn yields. Studying the genetic mechanism of maize lodging resistance-related traits has important guiding signif...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12Q1/6895C12Q1/6869G16B20/30
CPCC12N9/0044C12Q1/6895C12Q1/6869C12Y107/99004G16B20/30C12Q2600/13
Inventor 李鹏程汤潇王后苗吉伟东武婷婷徐暑晖杨泽峰徐辰武
Owner YANGZHOU UNIV
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