Preparation and application of double-ligand modified liver cancer targeting liposome

A technology targeting liposomes and dual ligands is applied in the directions of liposome delivery, medical preparations without active ingredients, and medical preparations containing active ingredients. Cells and other problems, to achieve the effect of reducing drug side effects, improving curative effect, and good tumor inhibition effect

Pending Publication Date: 2022-03-22
GUANGXI UNIV
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Problems solved by technology

[0003] Chemotherapy is often used in clinical treatment of liver cancer. Doxorubicin is a commonly used first-line antineoplastic drug, which is effective for a variety of solid tumors. It will damage normal

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  • Preparation and application of double-ligand modified liver cancer targeting liposome
  • Preparation and application of double-ligand modified liver cancer targeting liposome
  • Preparation and application of double-ligand modified liver cancer targeting liposome

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0033] refer to Figure 1-7 , screening of DSPE-PEG1000-TAT dosage in SP94 / TAT-DOX-LP;

[0034] The density of TAT modified on the surface of liposomes has a great influence on the cell-entry ability of the macromolecule carrier mediated. By exploring the effects of different TAT densities on the ratio of liposome cell uptake capacity and DOX encapsulation efficiency, we screened out Optimum TAT dosage;

[0035] Other material content in the fixed liposome, wherein the content of DSPE-PEG3400-SP94 is 0%, the amount of regulating DSPE-PEG1000-TAT accounts for 0.5%, 0.75%, 1%, 1.25% of the mol ratio of soybean lecithin (SPC) %, 1.5%, (burst) to prepare liposomes. An...

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Abstract

The invention discloses a preparation method and application of a double-ligand modified liver cancer targeted liposome. The preparation method comprises the following steps: dissolving soybean lecithin, cholesterol, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and distearoyl phosphatidyl ethanolamine-polyethylene glycol 1000 connected with TAT peptide with a proper amount of chloroform; carrying out reduced-pressure rotary evaporation on the dissolved solution to form a uniform hyaluronic membrane, and then carrying out vacuum drying; adding a proper amount of distearoyl phosphatidyl ethanolamine-polyethylene glycol 3400 which is connected with the SP94 peptide into the dried hyaluronic membrane; according to the invention, adriamycin is used as a model drug and is entrapped in the SP94 and TAT co-modified liposome, and after the liposome reaches the interior of liver cancer cells through a targeting effect, adriamycin is released, so that a good tumor inhibition effect is achieved, and the side effect of the drug is reduced; the liver cancer targeting ability and cytotoxicity of the constructed SP94 and TAT co-modified adriamycin liposome are evaluated, and a new strategy is provided for enhancing liver cancer drug delivery.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to the preparation and application of a liver cancer targeting liposome modified by double ligands. Background technique [0002] Liver cancer is a common malignant tumor clinically. my country is a country with a high incidence of liver cancer. It has become the second most fatal malignant tumor in my country and poses a serious threat to the life and health of Chinese citizens. Because liver cancer has fast growth and easy metastasis The characteristics of liver cancer make the treatment of liver cancer particularly difficult. [0003] Chemotherapy is often used in clinical treatment of liver cancer. Doxorubicin is a commonly used first-line antineoplastic drug, which is effective for a variety of solid tumors. It will damage normal physiological cells, resulting in greater toxic and side effects. Therefore, we propose the preparation and application of a double-ligan...

Claims

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Application Information

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IPC IPC(8): A61K47/69
CPCA61K47/6911A61K47/62A61K31/704A61P35/00
Inventor 吴昊天袁明清张佳佳
Owner GUANGXI UNIV
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