Super-resolution microscopic imaging device and method based on spatial light modulator

Abstract

The invention discloses a super-resolution microscopic imaging device and method based on a spatial light modulator, and belongs to the field of optical microscopic imaging. Comprising a light source, a polarization beam splitter prism, an excitation light path, a loss light path and a coaxial system, a polarization beam splitter prism is arranged in front of the light source, an excitation light path is arranged at an outlet in one side of the polarization beam splitter prism, and a loss light path is arranged at an outlet in the other side of the polarization beam splitter prism; the outlet side of the excitation light path is coupled into the coaxial system; an outlet of the loss light path is coupled into the coaxial system; an imaging system is arranged on one side of the coaxial system, and a sample to be detected is arranged on the other side of the coaxial system. The system has the beneficial effects that the resolution of the whole system can be adjusted by the vortex light beam adjusted in real time under the condition that the existing light path structure is not changed, and the system can be rapidly adjusted according to different application scenes; and the structure is simple, the resolution ratio is ultrahigh, and the resolution ratio is dynamically adjustable.

Description

technical field [0001] The invention relates to a super-resolution microscopic imaging device and method based on a spatial light modulator, belonging to the field of optical microscopic imaging. Background technique [0002] Due to the influence of the diffraction limit, the resolution of traditional optical microscopes is limited to about half a wavelength. In the past two decades, many super-resolution imaging techniques bypassing the optical diffraction limit have emerged through different methods. Among them, stimulated emission depletion microscopy (STED) suppresses the fluorescence molecules in the periphery of the fluorescence spot by introducing a beam of ring-shaped depletion light. In order to achieve the purpose of reducing the point spread function and realize super-resolution imaging. The invention of super-resolution microscopy technology is of great significance to the fields of biomedicine and materials science. However, the traditional STED system consists...

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Problems solved by technology

The invention of super-resolution microscopy technology is of great significance to the fields of biomedicine and materials science. However, the traditional STED system consists of an excitation optical path and a loss optical path. The two beams of light are coupled into the coaxial micro-scanning system. The entire STED The structure is huge, and the system resolution is inversely proportional to the overall microscopic scanning speed. In scenarios that require different resolutions, it is necessary to replace the optical components in the lost light. After replacing the components, the overall optical path needs to be adjusted on the coaxial axis, which is very inconvenient.

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