Covalent organic framework-platinum composite nano antibacterial agent for killing drug-resistant pathogenic bacteria

A covalent organic framework and nano-antibacterial agent technology, applied in the field of biomedicine, can solve the problems of sharp decline in catalytic antibacterial activity, restrictions on the practical application of PtNPs, and easy agglomeration

Pending Publication Date: 2022-04-08
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the extremely high surface energy of PtNPs, especially in biological matrices, their catalytic antibacterial activity drops sharply, which greatly limits the practical application of PtNPs.

Method used

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  • Covalent organic framework-platinum composite nano antibacterial agent for killing drug-resistant pathogenic bacteria
  • Covalent organic framework-platinum composite nano antibacterial agent for killing drug-resistant pathogenic bacteria
  • Covalent organic framework-platinum composite nano antibacterial agent for killing drug-resistant pathogenic bacteria

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Experimental program
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Effect test

Embodiment 1

[0015] 1. Experimental method

[0016] Using MRSA as a representative, the antibacterial properties of COF-PtNPs were studied. The bacteria were inoculated in about 4 mL of broth medium, placed in a shaking incubator at 37 ° C, and cultured at a speed of 220 rpm / min for about 3 h, by using a UV-visible spectrophotometer at 600 nm (OD 600nm ) to record the optical density, adjust the cell density of the cultured bacteria to 0.5OD 600nm . Then dilute the bacteria 20 times with normal saline, and add samples in the following groups: (1) Control 1: NaAc / HAc; (2) Control 2: H 2 o 2 ; (3) COF-PtNPs; (4) COF-Pt NPs+H 2 o 2 , the total volume of each tube is 400 μL, after mixing, incubate at 37°C for 1h, spread the bacterial suspension on the LB agar plate, and place it upside down in a constant temperature incubator at 37°C for about 18h. In addition, by measuring OD 600nm To detect the concentration of bacteria to quantify its antibacterial rate. OD of LB medium containing b...

Embodiment 2

[0021] 1. Experimental method

[0022] In order to evaluate the antibacterial effect of COF-PtNPs in vivo, male BALB / c mice (about 7 weeks old, about 20 g, 2 mice per group) were used to establish a mouse wound infection model. The mice were weighed and marked first, and then the mice were anesthetized with ether. After the hair on the back of the mice was shaved, a circular skin wound with a diameter of about 4.5 mm was produced on the back of each mouse, and 60 μL of MRSA (10 8 CFU / mL) suspension was placed on the wound. After infection, the mice were divided into 4 groups and treated with different treatments: (1) Control; (2) H 2 o 2 ; (3) COF-PtNPs; (4) COF-PtNPs+H 2 o 2 . H used in this study 2 o 2 The dose was 70 μL (250 μM) per site. The dose of nanozymes was 70 μL per site (5 μg / mL). Treatments were performed once a day, and wound photographs were taken for each group at each expected time point.

[0023] 2. Result analysis

[0024] With COF-PtNPs and COF-P...

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Abstract

The invention discloses a covalent organic framework-platinum composite nano-antibacterial agent for broad-spectrum treatment of pathogenic bacteria represented by methicillin-resistant staphylococcus aureus, which comprises the following steps: co-incubating covalent organic framework-platinum composite nano-antibacterial agents and methicillin-resistant staphylococcus aureus with different concentrations, and researching the antibacterial effect of the covalent organic framework-platinum composite nano-antibacterial agent and methicillin-resistant staphylococcus aureus. Experimental results prove that the composite nano antibacterial agent has an excellent bactericidal effect in vivo and in vitro, and the antibacterial agent has good biocompatibility. The covalent organic framework-platinum composite nano antibacterial agent disclosed by the invention is expected to play a role in clinical treatment of multidrug-resistant bacterial infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a covalent organic framework-platinum (CovalentOrganic Framework, COF; PlatinumNanoparticles, PtNPs) composite nano-antibacterial agent for killing drug-resistant pathogenic bacteria. Background technique [0002] Infectious diseases caused by bacteria are currently a major health problem facing the world. Represented by multidrug-resistant bacteria—Methicillin Resistant Staphylococcus Aureus (MRSA), MRSA is a common and highly infectious bacterium in clinical practice, and is one of the important pathogenic bacteria of hospital infection. It causes a series of organ-specific infections, the most common being skin and soft tissue infections, followed by invasive infections, such as osteomyelitis and pneumonia, which seriously threaten human health. The overuse or misuse of antibiotics will directly lead to the emergence of bacterial resistance. In the long run, they will bre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K33/243A61P31/04A61P17/02
Inventor 李孟遥张普母昭德王燚
Owner CHONGQING MEDICAL UNIVERSITY
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