Pea peptide with muscle building effect as well as preparation method, medicine and application of pea peptide

A pea peptide and action technology, applied in the field of peptides, can solve problems such as limiting the development of peptide drugs for the prevention and treatment of sarcopenia

Active Publication Date: 2022-04-12
中食都庆(山东)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the types of protein peptides with muscle-enhancing effects in pea have not be...

Method used

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  • Pea peptide with muscle building effect as well as preparation method, medicine and application of pea peptide
  • Pea peptide with muscle building effect as well as preparation method, medicine and application of pea peptide
  • Pea peptide with muscle building effect as well as preparation method, medicine and application of pea peptide

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preparation example Construction

[0052] The invention provides a preparation method of the pea peptide, comprising the following steps:

[0053] 1) enzymatically hydrolyzing pea protein under the action of alkaline protease to obtain the first enzymatic hydrolyzate;

[0054] 2) enzymatically hydrolyzing the first enzymolyzate under the joint action of papain and cellulase to obtain a second enzymolyzate;

[0055] 3) Pass the second enzymolysis solution through a 3000Da ceramic membrane after deactivating the enzyme, and pass the obtained filtered part through a 1KDa organic membrane to collect the components on the membrane to obtain pea peptide;

[0056] 4) subject the pea peptide to gel chromatography, and collect component S2 for 12-18 minutes;

[0057] 5) The component S2 was separated and purified by preparative liquid chromatography, and the component W5 was collected for 55-63 minutes;

[0058] 6) The component W5 was separated and purified by analytical liquid chromatography, and five polypeptide fr...

Embodiment 1

[0087] The production method of pea peptide, the steps are as follows:

[0088] Step 1. Add 10000L ion-free water to the enzymolysis tank, use NaOH with a concentration of 10mol / L to adjust the pH value to 9.0, add 10kg of alkaline protease, and continuously stir at a speed of 65 r / min during the process, and use steam to Raise the temperature to about 55°C.

[0089] Step 2. Prepare 700kg of pea protein for feeding, and ensure that the feeding is completed within 30 minutes. During the stirring process, after the feeding is completed, use NaOH with a concentration of 10mol / L to adjust the pH to 9.0, and continue to add 4kg of alkaline protease. Solution 3h.

[0090] Step 3. Add 1% papain (7.0kg) and 0.1% cellulase (0.7kg) after alkaline protease hydrolysis for 3 hours, and continue enzymatic hydrolysis for 2 hours.

[0091] Step 4. The temperature of the solution in step 3 is raised to 110° C. and kept for 5 minutes to inactivate the two proteases and cellulase.

[0092] St...

Embodiment 2

[0097] Isolation, purification and identification of muscle-enhancing activity of pea peptide

[0098] 1. Gel Chromatography

[0099] Take 10 g of pea peptide prepared in Example 1, add 200 ml of 2% acetic acid solution and stir to dissolve for 0.5 h. Centrifuge the pea peptide at 8000r / min, 15°C for 15min, filter through a filter head with a pore size of 0.45μm and set aside.

[0100] Separation of Sephadex G50 chromatographic column: First, equilibrate the column with ultrapure water to wash 4 column volumes before loading the sample, then wash the column with ultrapure water at 2.5mL / min for 2h, collect the fractions of the pea peptide after passing through the membrane according to the chromatographic peak and divide each The fractions were concentrated and freeze-dried in a vacuum dryer into powder. The separation conditions are: eluent is ultrapure water; elution time is 2h; elution rate: 2.5mL / min; chromatographic column size is 600 mm×25 mm; detection wavelength: 214...

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Abstract

The invention provides pea peptide with a muscle building effect as well as a preparation method, a medicine and application thereof, and belongs to the technical field of polypeptide. The pea protein is subjected to enzymolysis, and five polypeptides with an obvious muscle building effect are obtained through separation. In an in-vitro aging skeletal muscle cell test, changes of gene expression levels of regulatory pathways related to skeletal muscle cell proliferation and differentiation, cell apoptosis autophagy and protein synthesis and degradation are analyzed, and animal tests are carried out at the same time to research the muscle building effect and mechanism of the skeletal muscle cells, so that the five polypeptide sequences have remarkable muscle building effect; the polypeptide is expected to be developed into a novel polypeptide medicine for treating or preventing sarcopenia.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to a pea peptide with a muscle-enhancing effect and a preparation method, medicine and application thereof. Background technique [0002] Sarcopenia is a syndrome resulting from persistent loss of skeletal muscle mass, strength, and function. Skeletal muscle is the driving force of the human body movement system. The aging and atrophy of muscle is an important sign of human aging, which is very easy to cause fractures and joint damage. Sarcopenia is a disease related to age and aging. With the increase of age and the decrease of activity, the muscle content and strength of people will gradually decline, and the muscles of the body cannot maintain balance and agility. It will cause weakened mobility, make the body become sluggish, have poor balance, and fall more easily. Therefore, sarcopenia will increase the hospitalization rate and medical expenses of the elderl...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/14A61K38/08A61P21/00
CPCA61P21/06A61K38/168
Inventor 张钊张九勋张政李杰魏玮张西平裴中立谷帅牛立柱
Owner 中食都庆(山东)生物技术有限公司
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