A kind of pea peptide with muscle-enhancing effect and its preparation method, medicine and application

A technology of pea peptides and functions, applied in the field of peptides, can solve problems such as the development of peptide drugs that limit the prevention and treatment of sarcopenia

Active Publication Date: 2022-07-19
中食都庆(山东)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the types of protein peptides with muscle-enhancing effects in pea have not been clarified, which limits the development of peptide drugs for the prevention and treatment of sarcopenia

Method used

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  • A kind of pea peptide with muscle-enhancing effect and its preparation method, medicine and application
  • A kind of pea peptide with muscle-enhancing effect and its preparation method, medicine and application
  • A kind of pea peptide with muscle-enhancing effect and its preparation method, medicine and application

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preparation example Construction

[0052] The present invention provides a method for preparing the pea peptide, comprising the following steps:

[0053] 1) Enzymatic hydrolysis of pea protein under the action of alkaline protease to obtain the first enzymatic hydrolysis solution;

[0054] 2) Enzymolysis of the first enzymatic hydrolysis solution under the combined action of papain and cellulase to obtain a second enzymatic hydrolysis solution;

[0055] 3) Passing the second enzymatic hydrolysis solution after deactivating the enzyme through a 3000Da ceramic membrane, and filtering the lower part through a 1KDa organic membrane to collect the components on the membrane to obtain pea peptides;

[0056] 4) subjecting the pea peptide to gel chromatography to collect component S2 for 12-18 min;

[0057] 5) separating and purifying the component S2 by preparative liquid chromatography, and collecting the component W5 for 55 to 63 minutes;

[0058] 6) The component W5 was subjected to analytical liquid chromatograp...

Embodiment 1

[0087] The production method of pea peptide, the steps are as follows:

[0088] Step 1. Add 10000L deionized water to the enzymolysis tank, adjust the pH value to 9.0 by using NaOH with a concentration of 10mol / L, add 10kg alkaline protease, and continuously stir at a rotating speed of 65 r / min. The temperature was raised to about 55°C.

[0089] Step 2. Prepare 700kg of pea protein for feeding, ensure that the feeding is completed within 30min, and continuously stir during the process. After feeding is completed, use the NaOH with a concentration of 10mol / L to adjust the pH to 9.0, and continue to add 4kg of alkaline protease to continue the enzyme. Solution 3h.

[0090] Step 3. After 3 hours of alkaline protease hydrolysis, 1% papain (7.0kg) and 0.1% cellulase (0.7kg) were added, and the hydrolysis was continued for 2 hours.

[0091] Step 4. The solution in step 3 was heated to 110° C. and kept for 5 min to inactivate the two proteases and cellulase.

[0092] Step 5. Cool ...

Embodiment 2

[0097] Separation and purification of pea peptides and identification of muscle-enhancing activity

[0098] 1. Gel chromatography

[0099] Take 10 g of pea peptide prepared in Example 1, add 200 ml of 2% acetic acid solution, and stir to dissolve for 0.5 h. The pea peptide was centrifuged at 8000 r / min, 15° C., 15 min, filtered through a filter head with a pore size of 0.45 μm, and used for later use.

[0100] Sephadex G50 chromatographic column separation: First, equilibrate the column with ultrapure water for 4 column volumes and then load the sample, then flush the column with ultrapure water at 2.5mL / min for 2h, collect the pea peptides after passing through the membrane according to the chromatographic peaks. The fractions are concentrated and freeze-dried into powder with a vacuum dryer. The separation conditions are as follows: the eluent is ultrapure water; the elution time is 2h; the elution speed: 2.5mL / min; the size of the chromatographic column is 600 mm×25 mm; t...

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Abstract

The invention provides a pea peptide with muscle-enhancing effect and a preparation method, medicine and application thereof, belonging to the technical field of polypeptides. The present invention separates and obtains 5 polypeptides with obvious muscle-enhancing effect through enzymatic hydrolysis of pea protein. In vitro aging skeletal muscle cell test, by analyzing the changes in gene expression levels of skeletal muscle cell proliferation and differentiation, apoptosis and autophagy, and protein synthesis and degradation-related regulatory pathways, animal experiments were carried out on its muscle-enhancing effect and its mechanism. Studies have shown that the five polypeptide sequences have significant muscle-enhancing effects, and are expected to be developed as new polypeptide drugs for the treatment or prevention of sarcopenia.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to a pea peptide with muscle-enhancing effect and a preparation method, medicine and application thereof. Background technique [0002] Sarcopenia is a syndrome caused by the continuous loss of skeletal muscle mass, strength and function. Skeletal muscle is the driving force of the human body's movement system. The aging and atrophy of muscles are important signs of human aging, which are very easy to cause fractures and joint damage. Sarcopenia is a disease related to aging. With the increase of age and the reduction of activity, the muscle content and strength of people will gradually decline, and the muscles of the body cannot maintain balance and agility. It will cause a decrease in mobility, making the body sluggish, poor balance, and more likely to fall. Therefore, sarcopenia will increase the hospitalization rate and medical expenses of the elderly, serious...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/14A61K38/08A61P21/00
CPCA61P21/06A61K38/168
Inventor 张钊张九勋张政李杰魏玮张西平裴中立谷帅牛立柱
Owner 中食都庆(山东)生物技术有限公司
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