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Fluorescent microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi

A fluorescence microscope and Artemisia argyi gland technology, applied in fluorescence/phosphorescence, material analysis through optical means, measuring devices, etc., can solve the problems of affecting the observation of glandular hairs, the inability to observe the distribution of glandular hairs, and the difficulty of observing glandular hairs, so as to save reagents , low cost, and extremely easy to operate

Pending Publication Date: 2022-04-12
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

However, due to the dense distribution of T-type non-glandular hairs, and the extremely long apical cells (up to 2000 μm), intertwined and intertwined, it seems that only by observing the handle of T-type non-glandular hairs can effective observation and counting be done; Under the influence of autofluorescence such as chlorophyll, it is difficult to observe glandular hairs under a fluorescent microscope; at the same time, due to the interference of autofluorescence of non-glandular hairy top cells, the observation of glandular hairs is also affected, making it impossible to observe the lower surface of Artemisia argyi. Distribution of glandular and non-glandular hairs and complete counting

Method used

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  • Fluorescent microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi
  • Fluorescent microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi
  • Fluorescent microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi

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Embodiment 1

[0034]1. Remove fresh mature leaves from the mugwort plants to ensure no lesions.

[0035] 2. Wash the leaf surface with distilled water to remove dust and other impurities on the leaf surface, and dry the surface moisture.

[0036] 3. Then soak it in concentrated hydrochloric acid with a concentration of 30% for 5 minutes. Take out the leaves, rinse them with clean water, and let them dry.

[0037] 4. Take the above-mentioned leaves, and cut small pieces of 8mm square leaves on them, put them on the glass slide with the upper surface or the lower surface facing up, add a drop of distilled water, cover with a cover glass, and use an epifluorescence microscope under blue light. Or the glandular hairs can emit dazzling fluorescence under the excitation of green light, and the glandular hairs on the upper surface and the lower surface are respectively observed, photographed and counted ( figure 1 and figure 2 ).

[0038] 5. Take a weighing bottle and add 5ml of propylene gly...

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Abstract

The invention provides a fluorescence microscopic slide preparation technology for observing and counting glandular hair and non-glandular hair of folium artemisiae argyi, and relates to the technical field of plant microscopic identification. According to the method, the distribution condition of glandular hairs and non-glandular hairs can be clearly observed through fluorescent staining by utilizing the autofluorescence difference of the glandular hairs and the non-glandular hairs, and the observation and counting of the glandular hairs and the non-glandular hairs are respectively completed. The counting method provided by the invention has the advantages of small technical sampling amount, reagent saving, low cost, easiness in operation and high success rate, can easily achieve a satisfactory experimental purpose, and solves the major scientific problem which puzzles researchers for a long time.

Description

technical field [0001] The invention belongs to the technical field of microscopic identification of plants, and in particular relates to a fluorescent microscopic slide-making technique for observing and counting glandular hairs and non-glandular hairs of Artemisia argyi. Background technique [0002] Artemisia argyi is a plant belonging to the genus Artemisia in the family Compositae. There are many varieties of moxa, and it has been used all over the country several times. Different living environments have bred rich moxa resources, such as "Qi AI" in Qichun County, Hubei Province, "Bei AI" in Tangyin County, Henan Province, "Qi AI" in Anguo City, Hebei Province, and "Qi AI" in Ningbo City, Zhejiang Province. "Haiai" is the most representative variety. In summer, when the flowers are not in bloom, pick the leaves of the plant Artemisia argyi, remove impurities, and dry them in the sun to obtain the leaves of Artemisia argyi, a Chinese medicinal material. Artemisia argy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
Inventor 郭涛司彦坡回成程贾玉珍张明月欧阳臻张振强刘孟奇
Owner HENAN UNIV OF CHINESE MEDICINE
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