Fluorescent probe for marking and tracking cytoplasmic membranes and preparation method of fluorescent probe

Abstract

A fluorescent probe for marking and tracking cytoplasmic membranes and a preparation method of the fluorescent probe belong to the field of fine chemical engineering. According to the fluorescent probe, BODIPY is adopted as a substrate to react with carbazole aldehyde under nitrogen or argon protection, piperidine is adopted as a catalyst, and a 4-angstrom molecular sieve is adopted as a dehydrating agent; the reaction product reacts with a quaternization reagent to obtain fluorescent probe molecules. The fluorescent probe has a large emission wave length ranging from 600 to 700 nm, avoids the interference of autofluorescence of cells and reduces the optical damage on cells; the fluorescent probe has a bipolar structure, fluorescence quenching is caused when the fluorescent probe congregates in water, and the fluorescence is obviously improved when the fluorescent probe is embedded in the cytoplasmic membranes; the probe participates in biological processes together when positioned in the cytoplasmic membrane, and being free from toxic and side effects, the probe is suitable for microscopic observation of cellular processes related to the cytoplasmic membranes; the fluorescent probe has high synthesis efficiency and stable properties, is easy to preserve for a long term, and provides a visual and concise low-cost detection reagent for studying relevant processes of the cytoplasmic membranes.

Description

technical field [0001] The invention relates to a fluorescent probe for marking and tracking cell plasma membrane and a preparation method thereof, belonging to the field of fine chemical industry. Background technique [0002] The cell membrane is a barrier that prevents extracellular substances from freely entering the cell. It ensures the relative stability of the intracellular environment and enables various biochemical reactions to run in an orderly manner. It is also a channel for the exchange of information, matter and energy between cells and the surrounding environment, helping cells obtain the required substances and discharge metabolic waste. Fluidity is a basic characteristic of cell membranes, based on which cells perform a variety of physiological functions, such as the absorption, digestion and efflux of cell membranes through pinocytosis, phagocytosis or exocytosis , The substance inside. Therefore, real-time, dynamic, and long-term visual tracking of the ...

AI Technical Summary

Problems solved by technology

Currently, fluorophores for labeling and tracking cell membranes are mainly commercially available (Invitrogen TM ) FM series and Dil series, however, its stability to cell membrane markers is poor, which is not conducive to application, its own low photostability is not conducive to user operation, and its synthesis and preparation process is cumbersome, and it is difficult to store for a long time , resulting in high cost and high price, which is not conducive to widespread use

[0004] Although there have been corresponding studies and reports on some activity mechanisms of cells, it is still difficult for scientists to observe the more detailed activity mechanisms intuitively and in real time

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