Method and kit for detecting gout typing related genes
A kit and genetic technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve difficult problems such as gout typing, and achieve short operation time, low difficulty and high cost performance Effect
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[0050] Such as figure 1 Shown is the flowchart of the method for detecting gout typing-related genes according to the embodiment of the present invention;
[0051] The method of the present embodiment comprises the following steps:
[0052] The first step is to design specific primers for specific genes;
[0053] In the second step, specific PCR is used to amplify the target fragment containing the specific gene;
[0054] The third step, PCR product alkaline phosphatase treatment;
[0055] The fourth step is to perform a single base extension reaction;
[0056] The fifth step is to carry out resin purification treatment;
[0057] The sixth step is to detect and analyze the sequence of specific genes;
[0058] Wherein, the specific gene is: rs3775948, rs2231142, rs11231463 and rs504915 and combinations thereof.
[0059] The full names of the above genes are: rs3775948.GG of SLC2A9 / GLUT9, rs504915.AA of SLC22A12 / URAT1, rs2231142.GG of ABCG2, rs11231463.GG of OAT7.
[0060...
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