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Method and kit for detecting gout typing related genes

A kit and genetic technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve difficult problems such as gout typing, and achieve short operation time, low difficulty and high cost performance Effect

Pending Publication Date: 2022-05-10
查理高特(青岛)健康科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] The invention provides a new method and kit for detecting genes related to gout typing. By detecting the genotypes of several specific genomes, gout typing can be performed to solve the problem of rapid and accurate gout typing in the prior art. technical issues

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  • Method and kit for detecting gout typing related genes
  • Method and kit for detecting gout typing related genes
  • Method and kit for detecting gout typing related genes

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Embodiment Construction

[0050] Such as figure 1 Shown is the flowchart of the method for detecting gout typing-related genes according to the embodiment of the present invention;

[0051] The method of the present embodiment comprises the following steps:

[0052] The first step is to design specific primers for specific genes;

[0053] In the second step, specific PCR is used to amplify the target fragment containing the specific gene;

[0054] The third step, PCR product alkaline phosphatase treatment;

[0055] The fourth step is to perform a single base extension reaction;

[0056] The fifth step is to carry out resin purification treatment;

[0057] The sixth step is to detect and analyze the sequence of specific genes;

[0058] Wherein, the specific gene is: rs3775948, rs2231142, rs11231463 and rs504915 and combinations thereof.

[0059] The full names of the above genes are: rs3775948.GG of SLC2A9 / GLUT9, rs504915.AA of SLC22A12 / URAT1, rs2231142.GG of ABCG2, rs11231463.GG of OAT7.

[0060...

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Abstract

The invention provides a method and a kit for detecting gout typing related genes, and belongs to the field of gene detection methods. The method comprises the following steps: 1, designing a specific primer for a specific gene; 2, amplifying a target fragment containing a specific gene by utilizing specific PCR (Polymerase Chain Reaction); 3, carrying out alkaline phosphatase treatment on a PCR product; 4, carrying out a single base extension reaction; 5, carrying out resin purification treatment; 6, detecting and analyzing the sequence of a specific gene; wherein the specific gene is a combination of a plurality of genes selected from the group consisting of a plurality of genes selected from the group consisting of rs3775948 (SLC2A9), rs2231142 (ABCG2), rs11231463 (OAT7) and rs504915 (SLC22A12). According to the invention, by detecting a specific gene, whether a patient belongs to a uric acid excretion reduction type gout patient can be effectively judged according to a genotyping result of gene detection, so that a more reasonable treatment strategy can be formulated.

Description

technical field [0001] The invention relates to the manufacturing technology of medical diagnostic supplies, in particular to a method and kit for detecting genes related to gout typing, and belongs to the technical field of detection medicament manufacturing. Background technique [0002] Gout is a polygenic hereditary disease, and its occurrence is the result of the interaction and joint action of genetic factors and environmental factors. The combined effect of genes involved in uric acid excretion and metabolism will lead to increased synthesis of uric acid in the liver and / or decreased excretion of uric acid in the kidney, resulting in hyperuricemia, which is an important biochemical basis for the pathogenesis of gout. In addition, internal environmental factors including hypertension, hyperglycemia, lipid metabolism disorder, and food can induce or aggravate hyperuricemia and increase the risk of gout by affecting the synthesis and (or) excretion of uric acid, respecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/112C12Q2521/525C12Q2531/113C12Q2535/125C12Q2565/627
Inventor 李长贵孙明妹孙文艳
Owner 查理高特(青岛)健康科技有限公司
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