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Application of INPP5F in preparation of medicine for prognosis, diagnosis or prevention and treatment of liver cancer tissue tumors

A technology for liver cancer tissue and uses, applied in the field of biomedicine, can solve the problem of less research on malignant tumors, and achieve the effect of promoting proliferation

Pending Publication Date: 2022-05-13
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] More and more studies have confirmed that the abnormal expression of phosphatases can affect the regulation of tumorigenesis and development. Recent studies have found that the inositol polyphosphate 5-phosphatase family can also exhibit cancer-promoting effects during tumor progression, but its member INPP5F alone However, there are still few studies on its role in the occurrence and development of malignant tumors.
The role of INPP5F in hepatocellular carcinoma and the mechanism involved have not been reported yet

Method used

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  • Application of INPP5F in preparation of medicine for prognosis, diagnosis or prevention and treatment of liver cancer tissue tumors
  • Application of INPP5F in preparation of medicine for prognosis, diagnosis or prevention and treatment of liver cancer tissue tumors
  • Application of INPP5F in preparation of medicine for prognosis, diagnosis or prevention and treatment of liver cancer tissue tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 (Expression of INPP5F in liver cancer and its clinical significance)

[0086] In this embodiment, through searching and analyzing public databases Gepia and Oncomine, it is found that the expression of INPP5F is abnormally up-regulated in liver cancer, and the expression of INPP5F in hepatocellular carcinoma is detected by RT-PCR, immunohistochemistry and other experimental methods, and its expression is compared with clinical The clinical significance of INPP5F was analyzed by combining pathological and prognostic information. The specific operation is as follows:

[0087] 1 material

[0088] 1.1 Clinical tissue samples

[0089] Real-time fluorescence quantitative PCR: Human liver cancer tissue samples were obtained from the Department of Hepatobiliary Surgery of Sun Yat-sen Memorial Hospital, Sun Yat-sen University. The operation time was from April 2015 to February 2016, with a total of 88 cases; the samples were immediately put into cryopreservation tube...

Embodiment 2

[0170] Example 2 (INPP5F promotes liver cancer cell proliferation and aerobic glycolysis)

[0171] Example 1 verified that the expression level of INPP5F in liver cancer tissue was significantly higher than that in normal liver tissue at the histological level. Through statistical analysis with the clinicopathological and prognostic characteristics of the patient, it is believed that the expression level of INPP5F is related to the tumor size and tumor differentiation of the patient. The degree and survival time are closely related. This example will discuss the specific biological functions of INPP5F in hepatocellular carcinoma cells. Firstly, knock down and overexpress INPP5F in hepatocellular carcinoma cells; secondly, explore the effects of changes in the expression level of INPP5F on cell proliferation, invasion, metastasis and aerobic glycolysis in hepatocellular carcinoma; finally, through subcutaneous and in situ growth in mice The tumor model was used to verify the e...

Embodiment 3

[0351] Example 3 (Research on the mechanism of INPP5F promoting the proliferation of liver cancer cells)

[0352] Example 2 In vivo cell experiments verified that INPP5F protein can promote cell proliferation and aerobic glycolysis in hepatocellular carcinoma. The mouse subcutaneous xenograft model and liver orthotopic xenograft model showed that INPP5F can also promote liver cancer cells in vivo. proliferation. In this example, the transcriptome sequencing technology was used to clarify the expression changes of the downstream genes of INPP5F; at the same time, the interacting proteins of INPP5F were identified through protein spectrum analysis, and whether the target gene was involved in the process of INPP5F promoting the proliferation of liver cancer cells was further explored.

[0353] 1 material

[0354] 1.1 Main reagents and consumables

[0355]

[0356] The primer sequences involved are as follows:

[0357]

[0358] The antibodies and concentrations used in we...

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Abstract

The invention relates to the technical field of biological medicines, and discloses application of INPP5F in preparation of drugs for prognosis, diagnosis or prevention and treatment of liver cancer tissue tumors. The INPP5F is abnormally highly expressed in hepatocellular carcinoma tissues, the expression level of the INPP5F is closely related to the tumor size, the tumor differentiation degree and the accompanied liver cirrhosis, and the INPP5F has the capabilities of promoting proliferation of hepatocellular carcinoma cells, aerobic glycolysis and in-vivo tumor formation and is a novel nucleoplasm shuttle protein. The INPP5F gene or the INPP5F protein can be used as a marker for prognosis or diagnosis of the liver cancer, and has the potential of being applied to preparation of drugs or reagents for prognosis, diagnosis, treatment and / or prevention of the liver cancer.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, and more specifically, relates to the use of INPP5F for preparing drugs for the prognosis, diagnosis or prevention of liver cancer tissue tumors. Background technique [0002] Hepatocellular carcinoma is one of the most common malignant tumors worldwide, and its prevalence has increased rapidly in recent years. Current treatments for HCC are limited and far from satisfactory. At the same time, the recurrence and metastasis rate of hepatocellular carcinoma is high, and 60-70% of patients will have recurrence and metastasis 5 years after hepatocellular carcinoma resection. Therefore, exploring the factors affecting tumor growth, recurrence and metastasis and the regulatory mechanisms involved can provide new ideas for the treatment of HCC and improve the survival status of patients. [0003] Inositol polyphosphate 5-phosphatase F (Inositol polyphosphate 5-phosphatase F, INPP5F), also ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G01N33/573A61K45/00A61P35/00A61P1/16
CPCC12Q1/6886G01N33/57438G01N33/57484G01N33/573A61K45/00A61P35/00A61P1/16C12Q2600/158C12Q2600/118C12Q2600/136G01N2333/916
Inventor 周倩蕾周振宇肖治宇王捷
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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