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Capturing and library building integrated equipment

A technology for equipment and library components, applied in biochemical equipment and methods, biochemical instruments, biochemical cleaning devices, etc., can solve problems such as unfavorable purity and easy pollution, and achieve the effect of strong practicability and pollution reduction.

Pending Publication Date: 2022-06-07
上海何因生物科技有限公司
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Problems solved by technology

[0003] The establishment of existing gene banks mostly relies on the manual operation of technicians to manually transfer the raw materials between test tubes. This kind of manual operation is easy to cause pollution and is not conducive to improving the purity of the target. Based on this, a capture method is now provided. Integrated equipment for building database

Method used

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  • Capturing and library building integrated equipment
  • Capturing and library building integrated equipment
  • Capturing and library building integrated equipment

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Embodiment 1

[0027] see Figure 1-Figure 5 , In the embodiment of the present invention, an integrated device for capturing and building a library includes a protective casing 120 and an installation cavity 121 arranged inside the installation cavity 121. Inside the installation cavity 121 is a library building component for building a library. The library assembly includes at least one fixed seat 100, and a drug storage part 108 for storing drugs and a PCR part 103 for gene amplification are arranged in the middle position of the upper end of the fixed seat 100. The upper end of the fixed seat 100 is also provided with a The medicine in the medicine storage part 108 is transferred to the medicine liquid transfer assembly in the PCR part 103, so that the mechanized dispensing can be realized and completed in a sealed space, which effectively reduces the pollution problem caused by the traditional device. The protective shell 120 There is a control screen corresponding to the liquid transfe...

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Abstract

The invention discloses capturing and library building integrated equipment which comprises a protective shell and a mounting cavity formed in the protective shell, a library building assembly used for building a library is arranged in the mounting cavity, and the library building assembly comprises at least one fixing base; the middle position of the upper end of the fixed seat is provided with a medicament storage part for storing a medicament and a PCR part for gene amplification, and the upper end of the fixed seat is also provided with a liquid medicine transfer assembly for transferring the medicament in the medicament storage part into the PCR part. And the whole process is completed mechanically, manual intervention is not needed, pollution to the external environment is effectively reduced, and practicability is high.

Description

technical field [0001] The invention relates to the technical field of biotechnology equipment, in particular to an integrated equipment for capturing and building a library. Background technique [0002] At this stage, to determine the sequence of the target region by sequencing technology, it is often necessary to construct a library molecule. The method for constructing a library molecule generally includes the following steps: 1) Obtaining nucleic acid molecules containing the target region to be determined by PCR amplification; 2) In The two ends of the nucleic acid molecule containing the target region to be determined are respectively connected with different linker molecules to obtain a sequencing library. [0003] The establishment of the existing gene bank mostly relies on the manual operation of technicians, and the raw materials are manually transferred between test tubes. This kind of manual operation can easily cause pollution and is not conducive to improving ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00
CPCC12Q1/6806C12Q2531/113
Inventor 杨吉元李敏
Owner 上海何因生物科技有限公司
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