Method for improving development and reproduction of cotesia nitenella by utilizing metformin hydrochloride

Abstract

The invention belongs to the field of biological prevention and control of agricultural and forestry pests, and relates to a method for improving development and reproduction of cotesia abrotanoides by using metformin hydrochloride, which comprises the following steps: feeding adult cotesia abrotanoides populations with a honey aqueous solution containing metformin hydrochloride as supplementary nutrition, and breeding cotesia abrotanoides by using rice moth larvae impregnated with the metformin hydrochloride solution as hosts, according to the population number of the cotesia nilaparis, the soaked rice moth larvae in a fixed proportion are provided, the adult stage of the cotesia nilaparis is prolonged, and the reproductive capacity of the cotesia nilaparis is improved. According to the method, the rice moths are selected as intermediate hosts, the rice moth dipping treatment, adult period nutrition improvement and other dual measures are adopted, the adult period of the cotesia nitenella is prolonged, the improvement of the reproductive capacity of the cotesia nitenella is promoted, and the method has a good application prospect for promoting the propagation production quantification, planning and intensification of the cotesia nitenella. The invention provides a parasitic wasp source for releasing a large amount of cotesia brasiliensis to prevent and control palm pests, and plays a positive role in protecting the planting safety of the palm plant industry.

Description

technical field

[0001] The invention belongs to the field of biological control of agricultural and forestry pests, and relates to a method for artificially prolonging the adult stage and improving the reproductive capacity of the brown tapeworm wasp, and in particular to a method for feeding the brown tapeworm in the adult stage A method for prolonging the lifespan and reproductive capacity of adult worms containing a solution of metformin hydrochloride. Background technique

[0002] Palm plants are the iconic components of my country's tropical natural ecosystems, and are also important economic crops in the tropical regions of my country. Among them, the healthy development of palm plants such as coconut, betel nut and oil palm can not only maintain the stability of the natural ecological environment It also provides an important support for the current farmers' increase in production and income and future flexible employment. Coconut weed moth and red-veined ear borer ar...

Examples

Embodiment 1

[0025] Embodiment 1 (under the condition of not supplying the host rice moth, the female and male adult stages of the brown-banded carinae)

[0026] Collect the first-emergence adults of Brassella spp. with complete appendages, select 5 males and females with complete appendages, and put them into a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Use a medical cotton swab dipped in the adult nutrient solution to supplement nutrition for the adults of the brown tapeworm wasp (replace once every 1 to 3 days). No nutrient supplementation was used as control 1, and 20% honey aqueous solution (nutrient solution) was used to feed the adults of Brassostrea praecox as control 2. 10 to 20 repetitions were set up for each treatment group. The survival of female and male adults was observed and recorded twice a day, and the average number of days in the adult stage was calculated. The results are shown in Table 1.

[0027] Table 1 The female and male adult stages of C...

Embodiment 2

[0030] Embodiment two (under the condition of supplying the host rice moth, the female and male adult stages of the brown banded carinae)

[0031] Use 300-mesh gauze to seal the worm bag containing 8 middle-aged rice moth larvae, and put them into the concentration of 1 × 10. -3 mg / L, 1.5×10 -3 mg / L, 3×10 -3 mg / L, 6.25×10 -3 mg / L, 12.5×10 -3 mg / L, 25×10 -3 mg / L, 50×10 -3mg / L, 0.1 mg / L and 0.2 mg / L metformin hydrochloride aqueous solutions were immersed for 3 s, taken out to dry, and hung in a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Collect the first-emergence adults of the brown-banded moth wasp, and select 5 males and 5 males with complete appendages, and introduce them into the finger-shaped tube. Use a medical cotton swab to dip the adult nutrient solution of different concentrations of metformin hydrochloride, and then place it in a finger-shaped tube for supplementary nutrition of the adults of the brown tapeworm Brassella spp. The concent...

Embodiment 3

[0035] Example 3 (the number of parasitized rice moths and the number of progeny brown-striped moth bees)

[0036] Use 300-mesh gauze to seal the worm bag containing 5 middle-aged rice moth larvae, and put them into the concentration of 1 × 10. -3 mg / L, 1.5×10 -3 mg / L, 3×10 -3 mg / L, 6.25×10 -3 mg / L, 12.5×10 -3 mg / L, 25×10 -3 mg / L, 50×10 -3 mg / L, 0.1 mg / L and 0.2 mg / L metformin hydrochloride aqueous solutions were immersed for 3 s, taken out to dry, and hung in a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Collect the first-emergence adults of Brassella spp. with complete appendages, select 5 males and 5 males with complete appendages, and put them into the finger-shaped tube. Use a medical cotton swab to dip the adult nutrient solution of different concentrations of metformin hydrochloride, and then place it in a finger-shaped tube for supplementary nutrition of the adults of the brown tapeworm Brassella spp. The concentration of metformin hydrochl...

technical field

[0001] The invention belongs to the field of biological control of agricultural and forestry pests, and relates to a method for artificially prolonging the adult stage and improving the reproductive capacity of the brown tapeworm wasp, and in particular to a method for feeding the brown tapeworm in the adult stage A method for prolonging the lifespan and reproductive capacity of adult worms containing a solution of metformin hydrochloride. Background technique

[0002] Palm plants are the iconic components of my country's tropical natural ecosystems, and are also important economic crops in the tropical regions of my country. Among them, the healthy development of palm plants such as coconut, betel nut and oil palm can not only maintain the stability of the natural ecological environment It also provides an important support for the current farmers' increase in production and income and future flexible employment. Coconut weed moth and red-veined ear borer ar...

Examples

Embodiment 1

[0025] Embodiment 1 (under the condition of not supplying the host rice moth, the female and male adult stages of the brown-banded carinae)

[0026] Collect the first-emergence adults of Brassella spp. with complete appendages, select 5 males and females with complete appendages, and put them into a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Use a medical cotton swab dipped in the adult nutrient solution to supplement nutrition for the adults of the brown tapeworm wasp (replace once every 1 to 3 days). No nutrient supplementation was used as control 1, and 20% honey aqueous solution (nutrient solution) was used to feed the adults of Brassostrea praecox as control 2. 10 to 20 repetitions were set up for each treatment group. The survival of female and male adults was observed and recorded twice a day, and the average number of days in the adult stage was calculated. The results are shown in Table 1.

[0027] Table 1 The female and male adult stages of C...

Embodiment 2

[0030] Embodiment two (under the condition of supplying the host rice moth, the female and male adult stages of the brown banded carinae)

[0031] Use 300-mesh gauze to seal the worm bag containing 8 middle-aged rice moth larvae, and put them into the concentration of 1 × 10. -3 mg / L, 1.5×10 -3 mg / L, 3×10 -3 mg / L, 6.25×10 -3 mg / L, 12.5×10 -3 mg / L, 25×10 -3 mg / L, 50×10 -3mg / L, 0.1 mg / L and 0.2 mg / L metformin hydrochloride aqueous solutions were immersed for 3 s, taken out to dry, and hung in a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Collect the first-emergence adults of the brown-banded moth wasp, and select 5 males and 5 males with complete appendages, and introduce them into the finger-shaped tube. Use a medical cotton swab to dip the adult nutrient solution of different concentrations of metformin hydrochloride, and then place it in a finger-shaped tube for supplementary nutrition of the adults of the brown tapeworm Brassella spp. The concent...

Embodiment 3

[0035] Example 3 (the number of parasitized rice moths and the number of progeny brown-striped moth bees)

[0036] Use 300-mesh gauze to seal the worm bag containing 5 middle-aged rice moth larvae, and put them into the concentration of 1 × 10. -3 mg / L, 1.5×10 -3 mg / L, 3×10 -3 mg / L, 6.25×10 -3 mg / L, 12.5×10 -3 mg / L, 25×10 -3 mg / L, 50×10 -3 mg / L, 0.1 mg / L and 0.2 mg / L metformin hydrochloride aqueous solutions were immersed for 3 s, taken out to dry, and hung in a finger-shaped tube with a diameter of 3 cm and a length of 12 cm. Collect the first-emergence adults of Brassella spp. with complete appendages, select 5 males and 5 males with complete appendages, and put them into the finger-shaped tube. Use a medical cotton swab to dip the adult nutrient solution of different concentrations of metformin hydrochloride, and then place it in a finger-shaped tube for supplementary nutrition of the adults of the brown tapeworm Brassella spp. The concentration of metformin hydrochl...