Micro nucleic acid vacuum centrifugal concentration device and micro nucleic acid solution concentration method

Abstract

The invention provides a trace nucleic acid vacuum centrifugal concentration device and a trace nucleic acid solution concentration method. The trace nucleic acid vacuum centrifugal concentration device comprises a vacuum pot, an aerosol adsorption part, a centrifugal mechanism and a vacuumizing mechanism, an air exhaust hole is formed in the shell wall of the vacuum pan, the air exhaust hole communicates with the vacuumizing mechanism, and the vacuumizing mechanism is used for vacuumizing the internal environment of the vacuum pan; the centrifugal mechanism comprises a centrifugal rotor, the centrifugal rotor is rotatably arranged in the vacuum pot, and the centrifugal rotor is used for mounting a microcentrifuge test tube so as to centrifuge a trace nucleic acid sample in the microcentrifuge test tube; the aerosol adsorption part is arranged in the air exhaust hole and used for adsorbing nucleic acid aerosol in the internal environment of the vacuum pot. Under the conditions of preventing the trace nucleic acid samples from being excessively centrifuged and lost and preventing aerosol cross contamination among the nucleic acid samples, the physical and chemical properties of the nucleic acid are reserved, and the high-concentration trace nucleic acid solution sample meeting the human body and paraffin sample gene library quality standard is obtained.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid extraction, in particular to a vacuum centrifugal concentration device for micro-nucleic acid and a method for concentrating micro-nucleic acid solution. Background technique [0002] In the field of nucleic acid extraction, qualified fresh biological nucleic acid samples should reach 20ng / ul when the reaction volume is 1-3ul added to the system for downstream PCR reactions. Paraffin tissue samples have many short fragments of nucleic acid breakage. It is a difficult sample to extract high-quality nucleic acid. The concentration of micro-samples such as circulating tumor cells, cultured cells, exfoliated cells, punctured cells, and long-term preservation of paraffin tissue is 1-5ng. Samples below / ul are called trace nucleic acid samples, and samples with an extraction concentration of 0.1 ng / ul are called trace nucleic acid samples. Not long ago, trace or trace nucleic acid samples ...

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Problems solved by technology

[0007] The present invention provides a micro nucleic acid vacuum centrifugal concentration device and a micro nucleic acid solution concentration method, which are used to solve or improve at least one technical problem existing in the prior art, so as to eliminate cross-contamination between nucleic acid samples in the concentration process to a certain extent risk, obtaining a highly concentrated nucleic acid solution

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