Wolfberry 40K liquid phase chip and application thereof

A liquid phase chip and Lycium barbarum technology, applied in genomics, instruments, biochemical equipment and methods, etc., to achieve the effects of reducing costs, overcoming large amount of data, and simple data analysis

A liquid phase chip and Lycium barbarum technology, applied in genomics, instruments, biochemical equipment and methods, etc., to achieve the effects of reducing costs, overcoming large amount of data, and simple data analysis

CN114657238AActive Publication Date: 2022-06-24WOLFBERRY SCI INST NINGXIA ACAD OF AGRI & FORESTRY SCI
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  • Wolfberry 40K liquid phase chip and application thereof
  • Wolfberry 40K liquid phase chip and application thereof
  • Wolfberry 40K liquid phase chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A Lycium barbarum 40K liquid phase chip, comprising a Lycium barbarum 40K site probe mixture and a hybridization capture reagent; the Lycium barbarum 40K site probe mixture includes Lycium barbarum 40K SNP site probes and 165 Lycium barbarum SNP functional site probes , with an average of 2 capture probes per site.

Embodiment 2

[0044] The information of 5023 sites in the Lycium barbarum 40K SNP site in Example 1 is shown in Table 1; wherein, the starting site is the starting site corresponding to the capture probe, and the termination site is the termination corresponding to the capture probe. site.

[0045] Table 1. Information of 5023 loci in Lycium barbarum 40K SNP loci

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Embodiment 3

[0100] The site information of the 165 Lycium barbarum SNP functional sites in Example 1 is shown in Table 2; wherein, the start site is the start site corresponding to the capture probe, and the stop site is the stop position corresponding to the capture probe point.

[0101] Table 2. Site information of 165 Lycium barbarum SNP functional sites

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Abstract

The invention belongs to the technical field of gene chips, relates to a Chinese wolfberry 40K liquid phase chip and application, and in particular relates to a Chinese wolfberry 40K liquid phase chip which is developed by utilizing a targeted capture sequencing technology and is used for genotyping a Chinese wolfberry genome. The lycium barbarum 40K liquid phase chip comprises a lycium barbarum 40K site probe mixed solution and a hybridization capture reagent, the Chinese wolfberry 40K site probe mixed solution comprises a Chinese wolfberry 40K SNP site probe and 165 Chinese wolfberry SNP functional site probes, and two capture probes are arranged at each site on average. The Chinese wolfberry 40K liquid phase chip overcomes the defect of high application cost of genetic diversity analysis, QTL positioning and GWAS analysis of Chinese wolfberry in scientific research, solves the problem that no applicable product exists in Chinese wolfberry molecular assisted breeding and whole genome selective breeding, and accelerates the Chinese wolfberry research and breeding process.

Description

technical field [0001] The invention belongs to the technical field of gene chips, relates to a Lycium barbarum 40K liquid phase chip and its application, in particular to a Lycium barbarum 40K liquid phase chip developed by using a target capture and sequencing technology for genotyping the Lycium barbarum genome. Background technique [0002] Traditional molecular markers such as restriction fragment length polymorphisms, simple sequence repeats, etc., cannot meet the requirements of modern breeding due to the small number of genome distributions, cumbersome operation process, and low throughput. Single nucleotide polymorphisms (SNPs) have the characteristics of large number, wide distribution, easy rapid and large-scale screening, convenient genotyping, and relatively low price, and are widely accepted and used by scientific researchers. [0003] At present, in the gene chip technology used for SNP locus typing, the traditional solid-phase chip is based on the complementa...

Claims

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Application Information

Patent Timeline
24 Jun 2022
Publication
CN114657238A
IPC
C12Q1/6874; C12Q1/6895; G16B20/30
CPC
C12Q1/6874; C12Q1/6895; G16B20/30; C12Q2600/13; C12Q2600/156; C12Q2535/122; C12Q2565/501
Inventors
赵建华; 曹有龙