Breeding method by changing DNA molecule and surrounding molecule environment

A DNA molecular and environmental technology, applied in the field of breeding, can solve the problems of unclear expression control system of reverse transcription gene program, no permanent function of reverse transcription mechanism, etc.

Pending Publication Date: 2022-07-29
湖南袁禾农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although Xu Yili put forward the theory known as the original virus hypothesis later, but the two views are consistent, but until today, people have not found a permanent function of the reverse transcription mechanism of higher organisms, about the reverse transcription gene The control system for the expression of the program is not yet clear

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  • Breeding method by changing DNA molecule and surrounding molecule environment
  • Breeding method by changing DNA molecule and surrounding molecule environment
  • Breeding method by changing DNA molecule and surrounding molecule environment

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Embodiment 1

[0086] High-light-efficiency rice breeding: At present, there are more than 60 global collaborative research groups supported by the Bill Gates Foundation participated by Zhu Xinguang, with an annual scientific research funding of 200 million pounds; the research team led by Academician Yuan Longping, led by Duan Meijuan The key genes of the C4 pathway of other high-light-efficiency crops were transferred into rice through transgenic technology, and it was found that yields were not increased but decreased; years of research by Zhu Xinguang and his American collaborators have not yielded results.

[0087] Design of the method: Do not take the road of cloning all the genes of hybrid rice, because of this method of fixing heterosis, all previous experiments have failed. We adopt a combination of cytoplasmic introduction and DNA modification, focusing on inducing epigenetic variation, that is, by changing cytoplasmic components, so as to achieve the goal of reprogramming the contr...

Embodiment 2

[0103] 1. Declining cytoplasm

[0104] The method is to plant the tillers of rice for 20 generations. The method is to transplant the hybrid rice Indica You 63 seedlings and let them tiller. When each tiller reaches 3-5 leaves, the mother is removed and transplanted, and the transplanted plants are repeated. In three years and 20 generations, the seed material of the original hybrid rice Indica You 63, which was cloned for 20 generations and vegetatively propagated for 20 generations, was obtained.

[0105] In multi-generation asexual reproduction, due to the occurrence of some gene knockouts or the loss of active components in the cytoplasm due to polarization, the asexual generations of the 20-generation asexual meristems have weak physique and serious decline.

[0106] Indica You 63 cloned seeds obtained in this way will undergo strong changes after corn cytoplasmic introduction. For example, the hybrid rice Indica You 63 was originally an indica rice, but the Guxianyou 63 ...

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Abstract

The invention discloses a breeding method by changing DNA (deoxyribonucleic acid) molecule and surrounding molecule environment, which introduces micro-injury cytoplasm introduction, living tissue culture, grafting and multicellular chimera technologies on the basis of traditional sexual and asexual hybridization, so that DNA genetic gene change and epigenetic (control of DNA expression) change are kept coordinated and consistent, and the breeding efficiency is improved. Therefore, the purposes of stable nuclear-cytoplasmic symbiotic relationship and co-construction of nuclear-cytoplasmic functions are achieved. Meanwhile, a cell reverse transcription gene switch is frequently turned on by using a stress activation method in the nucleoplasm double cross breeding process, so that the structure change of DNA molecules in cell nucleuses can be changed along with the change of cytoplasm components, and the aims of comprehensively repairing and reforming genes are fulfilled.

Description

【Technical field】 [0001] The invention relates to the field of breeding, in particular to a breeding method by changing DNA molecules and surrounding molecular environments. 【Background technique】 [0002] Cellular chimeric organisms are a natural phenomenon that include: [0003] 1. Genetically used to refer to individuals with mosaic or mixed performance of different genetic traits. The meaning of immunology refers to the coexistence of two or more cell lines with different chromosomal compositions in an organism, which can tolerate each other, do not produce rejection, and are in a chimeric state with each other. [0004] 2. One of the types of chromosomal abnormalities. An individual composed of cell lines from different zygotes. Also called heterozygous chimera. Individuals formed from cell lines that develop from the same zygote into different karyotypes are called homologous mosaics (mosaic), also known as mosaics. A common chimera in animals is the bovine freema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01G2/30A01C1/00
CPCA01H1/02A01G2/30A01C1/00
Inventor 胡爱生谢常明
Owner 湖南袁禾农业科技有限公司
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