34 results about "Dna genetics" patented technology

The invention belongs to the field of microbial genetic engineering, and relates to a homologous recombination system in gram-negative bacteria, in particular to a klebsiella pneumoniae homologous recombination system and application thereof. Comprising a pBBR1 replication starting point, a kanamycin resistance gene, a rhamnose inducible promoter and a klebsiella pneumoniae source homologous recombination operon or a mutant of the klebsiella pneumoniae source homologous recombination operon. According to the invention, self homologous recombinant protein of klebsiella pneumoniae is excavated, and expression plasmids of the homologous recombination system of klebsiella pneumoniae are constructed, so that the application of the homologous recombination system of klebsiella pneumoniae in genome DNA genetic modification of short homologous arm mediated homologous recombination in klebsiella pneumoniae and other klebsiella pneumoniae is realized; or gene knockout and insertion are carried out on a bacteriophage genome.