Preparation method and application of isovitexin

A technology of isovitexin and an extraction method, applied in the field of preparation of isovitexin, can solve problems such as undiscovered isovitexin, and achieve the effects of high purity, clear chemical structure and good yield

Pending Publication Date: 2022-07-29
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of isovitexin in the clinical treatment of alcoholic fatty liver.

Method used

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  • Preparation method and application of isovitexin
  • Preparation method and application of isovitexin
  • Preparation method and application of isovitexin

Examples

Experimental program
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Effect test

preparation example Construction

[0025] In a specific embodiment, first relate to the preparation method of coriander flavonoid glycoside monomer isovitexin, and its steps are as follows:

[0026] 1) First coriander is extracted by heating under reflux with ethanol solution, and ethanol is recovered by filtration to obtain ethanol crude extract; the ethanol crude extract is extracted with petroleum ether, chloroform and ethyl acetate successively, until the ethyl acetate solution is colorless Until transparent, the ethyl acetate extract was collected, concentrated and dried to obtain ethyl acetate extract;

[0027] 2) The ethyl acetate extract was dissolved in methanol, filtered through a microporous membrane, the filtrate was concentrated, the obtained powder was recrystallized with methanol, the obtained fraction was applied to a silica gel column, gradient elution was performed, and the same components were collected by thin layer chromatography. Deliquoring, then applied to silica gel column, gradient elu...

Embodiment 1

[0031] Embodiment 1, the preparation of isovitexin

[0032] 1) After fully pulverizing 10 kg of dried coriander, add 90 L of 75% ethanol aqueous solution, extract under reflux for 3 times, filter and combine the ethanol extracts for 3 times, and recover ethanol in vacuum to obtain a crude ethanol extract.

[0033] 2) The ethanol crude extract is extracted with petroleum ether, chloroform and ethyl acetate successively, and the volumes of the three are respectively 1 / 3, 3 / 3 and 4 / 3 of the volume of the ethanol crude extract, and each extraction is performed 3 times until Until the ethyl acetate solution became colorless and transparent, the ethyl acetate extract was collected, concentrated and dried to obtain an ethyl acetate extract.

[0034] 3) Dissolve the ethyl acetate extract in methanol and filter with a microporous membrane (0.45 μm); the filtrate is concentrated to obtain powder, which is then recrystallized with methanol to obtain fractions; and then applied to a silic...

Embodiment 2

[0036] Embodiment 2, the preparation of isovitexin

[0037] 1) After fully pulverizing 10 kg of dried coriander, add 90 L of 70% ethanol aqueous solution, extract under reflux for 3 times, filter and combine the ethanol extracts for 3 times, and recover ethanol in vacuum to obtain a crude ethanol extract.

[0038] 2) The ethanol crude extract is extracted with petroleum ether, chloroform and ethyl acetate successively, and the volumes of the three are respectively 1 / 4, 4 / 3 and 3 / 2 of the volume of the ethanol crude extract, and each extraction is performed 4 times until Until the ethyl acetate solution became colorless and transparent, the ethyl acetate extract was collected, concentrated and dried to obtain an ethyl acetate extract.

[0039]3) Dissolve the ethyl acetate extract in methanol and filter with a microporous membrane (0.22 μm); the filtrate is concentrated to obtain powder, which is then recrystallized with methanol to obtain fractions; and then applied to a silica...

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Abstract

The invention discloses a preparation method and application of isovitexin. The method comprises the following steps: 1) heating, refluxing and extracting lawn pennywort herb with an ethanol solution to obtain an ethanol crude extract; sequentially extracting the ethanol crude extract with petroleum ether, chloroform and ethyl acetate, concentrating and drying to obtain an ethyl acetate extract; and 2) dissolving the ethyl acetate extract in methanol, filtering with a microporous filter membrane, concentrating the filtrate, recrystallizing the powder with methanol, loading fractions on a silica gel column, carrying out gradient elution and thin-layer chromatography to collect eluents of the same components, loading on the silica gel column, carrying out gradient elution and thin-layer chromatography to collect the eluents of the same components, and recrystallizing with methanol. According to the application, isovitexin is applied to preparation of the medicine for preventing and treating the alcoholic fatty liver, and the protection mechanism of isovitexin is anti-inflammatory and anti-oxidative stress. The invention provides a preparation process of isovitexin with high purity and good yield, and provides a novel compound with a clear structure and high efficiency for treating alcoholic fatty liver.

Description

technical field [0001] The invention relates to the technical field of natural extracts, in particular to a preparation method and application of isovitexin. Background technique [0002] Coriander (Hydrocotyle sibthorpioides Lam.) is a plant of the genus Umbelliferae, and has a long history as a common folk medicine in my country. The medicinal name of coriander first appeared in Volume 26 of "Ji Bei Qian Jin Yao Fang" in the Tang Dynasty. Sun Simiao said in the book: "A small grass that grows in warm and humid places near water channels and grows in winter. Its shape is similar to coriander. Also known as chicken intestine dish, it can treat hemorrhoids, and it is called coriander." This plant has the functions of clearing away heat and detoxification, diuresis and swelling, and dampness and yellowing. It is mainly used for the treatment of jaundice, dysentery, edema, stranguria, eyesight Nebula, sore throat, carbuncle sore, herpes zoster, bruises, various ringworms, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04A61P1/16
CPCC07D407/04A61P1/16
Inventor 苏宏梅韦锦斌朱武嫦黄瑀莘陈思韵韦园园丘岳石炎夏龙妍
Owner GUANGXI MEDICAL UNIVERSITY
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