Heart failure marker multi-parameter detection device and method based on smart phone

Abstract

The invention discloses a heart failure marker multi-parameter detection device and method based on a smart phone. The device comprises a fluorescence immunoassay test strip and a fluorescence detection instrument. The fluorescence immunoassay test strip is composed of a PVC base plate, a sample pad, a nitrocellulose membrane (NC membrane) and a water absorption pad. The fluorescence detection instrument is composed of a smart phone, a top plate, a darkroom, an instrument bottom plate, a lens, an optical filter, an optical filter clamping groove, an ultraviolet LED, an LED support, a power supply and a power supply charging circuit. A slot is formed in the rear part of the fluorescence detection instrument, a smart phone card slot is formed in the top plate, a fluorescence immune test strip is inserted from the slot, a smart phone is inserted from the smart phone card slot, and the smart phone takes a picture of the test strip and analyzes and processes a collected image. According to the invention, the detection limit of the BNP is 0.03 ng / mL, and the detection range is 0.1-7.5 ng / mL; the detection limit of the NT-proBNP is 0.095 ng / mL, and the detection range of the NT-proBNP is 0.1 to 7.5 ng / mL; the detection limit of ST2 is 4.492 ng / mL, and the detection range is 5-400 ng / mL.

Description

technical field [0001] The invention relates to the fields of immune sensors, biological immune technology, nanomaterials, image processing and the like, in particular to a multi-parameter detection device and method for heart failure markers based on smart phones. Background technique [0002] Quantum dots (QDs), as a new type of fluorescent labeling materials, are characterized by their tunable emission spectrum, large Stokes shift, strong photostability, broad excitation spectrum and narrow emission spectrum, and biological phase. It is widely used to improve the detection sensitivity of immunochromatography due to its advantages of good capacitance and long fluorescence lifetime. Quantum-dot submicrobeads (QBs) further enhance the fluorescence intensity by encapsulating a large number of quantum dots in polymer microspheres, and further enhance the optical and solution stability of QBs due to the encapsulation of the polymer shell. Therefore, it is often used as an anti...

AI Technical Summary

Problems solved by technology

At present, large-scale automatic biochemical analyzers are usually used for the detection and analysis of heart disease markers in blood. This kind of instrument is bulky, expensive and requires professional operation, which cannot meet the needs of instant and on-site detection.

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