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Extraction refining method for aloe-polysaccharide

An aloe vera polysaccharide and refining method technology, applied in the field of aloe vera polysaccharide extraction and refining, can solve the problems such as no known aloe vera polysaccharide extraction and refining method, and achieve the effect of simple extraction and refining method

Inactive Publication Date: 2004-06-30
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many pharmacological effects of aloe (such as the effect on the immune system, anti-free radical effect, anti-radiation effect, anti-gastric ulcer, promotion of wound healing, etc.) are related to the polysaccharides contained in aloe vera, but there is no extraction and refining method for aloe polysaccharides

Method used

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  • Extraction refining method for aloe-polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the processing of macroporous resin

[0018] ①Pretreatment of macroporous resin

[0019] The used resin model of the present invention has AB-8, X-5 type, H107 type, S-8 type, D3520 type, D4006 type, D4020 type, NKA-9 type (above is Tianjin Nankai University chemical plant production), XAD-2 Type, XAD-3 type, HP type (the above is produced by Tianjin Rubber Factory), SIP1300 type, SIP1400 type, M3-type (the above is produced by Shanghai Pharmaceutical Industry Research Institute), ABD-4 type, DM-130 type (above Produced for Jining Shandong Lukang Pharmaceutical Co., Ltd.), D 101 Type (produced by Tianjin Pesticide Factory), 1300-1 type, 1300-66 type (produced by Yangzhou Pharmaceutical Factory), 100 type, 500 type, 600 type (produced by Cangzhou Encheng Chemical Co., Ltd.), etc. The processing principles and steps for these resins are very similar. Hereby take the AB-8 type macroporous adsorption resin as an example to introduce as follows:

[0020] Th...

Embodiment 2

[0024] Embodiment 2: the preparation of aloe crude polysaccharide

[0025] Weigh 300g of fresh aloe leaves, cut into small pieces, add 3500ml of distilled water, extract in a water bath at 40-80°C for 2 hours, and extract three times. After letting cool, filter the residue with gauze, concentrate the combined extract to 300-800ml, centrifuge, add 95% ethanol to the supernatant, so that the ethanol content in the solution reaches 50%-80%, precipitate polysaccharides, and vacuum-dry. That is to obtain the crude polysaccharide of aloe vera.

Embodiment 3

[0026] Embodiment 3: Aloe vera crude polysaccharide refining method

[0027] 40g of prepared aloe crude polysaccharide is dissolved in 1000ml distilled water, and 20%~50% trichloroacetic acid solution or other organic acid solutions (for example, trifluoroacetic acid etc.) are added, so that the final concentration of trichloroacetic acid reaches 2%~6 %, after standing overnight at 4°C, the precipitate was removed by centrifugation. Concentrate the supernatant to 200-500ml, and slowly add the supernatant to the macroporous resin column bed with a dropper (the macroporous resin can be AB-8 type, X-5 type, H107 type, S-8 type , D3520, D4006, D4020, NKA-9, XAD-2, XAD-3, HP, SIP1300, SIP1400, M3-, ABD-4, DM-130, D 101 type, 1300-1 type, -66 type, 100 type, 500 type, or 600 type, etc., the refining effect is the same). Open the stopcock and at the same time begin to collect the effluent. After the sample solution completely enters the resin column bed, rinse it with distilled wa...

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Abstract

The method for extracting and refining aloe polysaccharide includes the following steps: uctting fresh leaf of aloe into small block, extracting with water at 40-80 deg.C, filtering extract, centrifugation and taking out supernatant fluid, adding 95% ethyl alcohol in the supernatant fluid, making the ethyl alcohol content be up to 50-80%, precipitating crude aloe polysaccharide, dissolving said crude aloe polysaccharide in water, adding 20-50% trichloroacetic acid or trifluoroacetic acid to make end concentration of trichloroacetic acid or trifluoroacetic acid be up to 2-6%, standing still, then centrifugation to remove precipitate to obtain secondary supernatant fluid, feeding it into macroporous resin column bed and using water to make elution, concentrating and adding 95% ethyl alcohol, precipitating polysaccharide, freeze-drying so as to obtain refined aloe polysaccharide.

Description

1. Technical field [0001] The present invention relates to a plant from the genus Aloe [for example: Aloe vern var.chinensis, Aloe barbadensis, Aloe arborescens, Aloe ferox, Aloe socotrina ), Aloe saponaria (Aloe saponaria), etc.] in the method of extracting and separating a kind of polysaccharide. 2. Background technology [0002] Aloe is a plant with high medicinal value. Modern pharmacological research shows that it has a wide range of pharmacological activities, such as causing diarrhea (see: Encyclopedia of Chinese Medicine Shanghai Science Press, 1986: 1076), anti-tumor (see: Phytotherapy Research , 1993 Jul, 7, s43-s47), antibacterial and anti-inflammatory (Glycobiology, 1995 Jan, 5, 39-44), promoting immunity (Neuroscience Letters, 1997 Nov, 238(1-2), 65-68), promoting wound healing (see: Journal of Ethnopharmacology, 1998 Jan, 59(3), 195-201), anti-radiation (see: Immunopharmacology, 1997 Oct, 37(2-3), 153-162), anti-gastric ulcer (see: The Lancet , 1999 Jul, 354(...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 谭仁祥徐琛罗兰李洋戈惠明
Owner NANJING UNIV
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