Use of krev alcohol in preparing tyrosinase inhibitor

A kind of technology of clevisol and tyrosinase, which is applied in the field of clevisol to achieve the effect of simple extraction and purification method

Inactive Publication Date: 2010-01-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Endophytic fungi of mangrove plants are important members of marine fungi. These microorganisms can produce many active metabolites that are the same as or different from those of host plants, including some biologically active substances that have never been found in terrestrial microorganisms. It is an important resource of tyrosinase inhibitors, but there are few reports on the research and development of acetylcholinesterase inhibitors from such fungi.

Method used

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  • Use of krev alcohol in preparing tyrosinase inhibitor
  • Use of krev alcohol in preparing tyrosinase inhibitor
  • Use of krev alcohol in preparing tyrosinase inhibitor

Examples

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Effect test

Embodiment 1

[0035] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 200g and put it into 1L 50% seawater, boil for 30min, filter, and use 50% seawater for the filtrate (seawater and tap water by volume) Add glucose 20g, agar 2.0g in ratio mixing) be settled to 1000ml). Seed culture medium formula: add 20g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0036] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 25°C for 7 days. Shake flask culture: Prepare the medium according to the formula of the seed medium, pack into 500 E...

Embodiment 2

[0040] A. Medium formula: slant medium formula: per liter of potato juice (peel the potato and cut it into small pieces, take 300g and put it in 1L 100% seawater, boil for 30min, filter, and dilute the filtrate to 1000ml with 100% seawater) Add 30 g of glucose and 3.0 g of agar. Seed medium formula: add 30g of sucrose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0041] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 28°C for 5 days. Shake flask culture: Prepare the medium according to the above seed medium formula, pack it into Erlenmeyer flasks (100ml / bottle), sterilize (121°C, 30min), insert t...

Embodiment 3

[0045] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 150g and put it into 1L 20% seawater, boil for 30min, filter, use 20% seawater for the filtrate (seawater and tap water by volume) Add glucose 15g, agar 1.5g in ratio mixing) to 1000ml). Seed culture medium formula: add 15g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0046] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarf clavus CCTCC NO: M206063, was transferred to the slant medium and cultured at 32°C for 5 days. Shake flask culture: Prepare medium according to the above seed medium formula, pack into 500 Erlenmeyer flask...

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Abstract

Gram Liewei in the preparation tyrosinase inhibitor's application relates to one kind of gram Liewei to be mellow mellowly, which provides one kind to originate from mangrove forest endogenous fungus secondary metabolite gram Liewei mellowly in the preparation tyrosinase inhibitor application. The preparation method is: dwarf rods Aspergillus BYY-1 seed culture; submerged fermentation; broth filter strains; concentrated filtrate decompression after repeatedly using organic solvent extraction, collection and decompression organic phase was concentrated extracts; Extracts of the silica gel column chromatography and recrystallization obtained after refining grams Levy alcohol. The pharmacological experiments have confirmed that the compound significantly inhibited the activity of tyrosine, can be used for preparation of tyrosinase inhibitors and applied to pharmaceuticals, cosmetics and food. It may obtain through the fermentation system, raw material is rich, moreover the extraction purification method is simple, suits the formalization production.

Description

technical field [0001] The present invention relates to a kind of crevetol, especially relate to a kind of endophytic fungus derived from mangroves-Aspergillus dwarf clavula BYY-1 (A.clavatonanicus) fungus metabolite crevetol as tyrosinase inhibitor in medicine products, cosmetics and food applications. Background technique [0002] Tyrosinase widely exists in humans, animals, plants and microorganisms, and is a Cu 2+ metalloenzymes as cofactors. This enzyme has the dual functions of oxygenase and oxidase, plays a key role in the process of melanin synthesis, and is the rate-limiting enzyme in the process of melanin synthesis. It catalyzes the hydroxylation of monophenols to diphenols and the oxidation of diphenols to quinones; quinones form the final reaction product melanin under non-enzymatic conditions. The activity of tyrosinase is closely related to the amount of melanin synthesis, and increased activity in the human body will lead to pigmentation diseases such as c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26A61K31/122A61P17/00C12R1/66
Inventor 沈月毛黄珊珊郑忠辉杜希萍黄耀坚宋思扬
Owner XIAMEN UNIV
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