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Use of krev alcohol in preparing antioxidation agent

A technology of crevetol and antioxidant, applied in the field of crevetol, can solve the problem of finding antioxidants, and achieve the effect of simple extraction and refining method and significant antioxidant effect

Inactive Publication Date: 2010-01-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Endophytic fungi of mangrove plants are important members of marine fungi. These microorganisms can produce many active metabolites that are the same as or different from those of host plants, including some biologically active substances that have never been found in terrestrial microorganisms. An important resource of antioxidants, but there are few reports on the research and development of antioxidant acetylcholinesterase inhibitors from such fungi

Method used

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  • Use of krev alcohol in preparing antioxidation agent
  • Use of krev alcohol in preparing antioxidation agent
  • Use of krev alcohol in preparing antioxidation agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 200g and put it into 1L 50% seawater, boil for 30min, filter, and use 50% seawater for the filtrate (seawater and tap water by volume) Add glucose 20g, agar 2.0g in ratio mixing) be settled to 1000ml). Seed culture medium formula: add 20g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0035] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 25°C for 7 days. Shake flask culture: Prepare the medium according to the formula of the seed medium, pack into 500 E...

Embodiment 2

[0039] A. Medium formula: slant medium formula: per liter of potato juice (peel the potato and cut it into small pieces, take 300g and put it in 1L 100% seawater, boil for 30min, filter, and dilute the filtrate to 1000ml with 100% seawater) Add 30 g of glucose and 3.0 g of agar. Seed medium formula: add 30g of sucrose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0040] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 28°C for 5 days. Shake flask culture: Prepare the medium according to the above seed medium formula, pack it into Erlenmeyer flasks (100ml / bottle), sterilize (121°C, 30min), insert t...

Embodiment 3

[0044] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 150g and put it into 1L 20% seawater, boil for 30min, filter, use 20% seawater for the filtrate (seawater and tap water by volume) Add glucose 15g, agar 1.5g in ratio mixing) to 1000ml). Seed culture medium formula: add 15g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0045] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarf clavus CCTCC NO: M206063, was transferred to the slant medium and cultured at 32°C for 5 days. Shake flask culture: Prepare medium according to the above seed medium formula, pack into 500 Erlenmeyer flask...

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Abstract

Gram Liewei in the preparation oxidation inhibitor's application, relates to one kind of gram Liewei to be mellow mellowly and provides one kind to originate from the mangrove forest endogenous fungus secondary metabolite gram Liewei mellow preparation method and in the preparation oxidation inhibitor application. The preparation method is: dwarf rods Aspergillus BYY-1 seed culture; submerged fermentation; broth filter strains; concentrated filtrate decompression after repeatedly using organic solvent extraction, collection and decompression organic phase was concentrated extracts; Extracts of the silica gel column chromatography and recrystallization obtained after refining grams Levy alcohol. The pharmacological experiments confirmed that the compounds have significant antioxidant role of antioxidants can be used and applied in pharmaceuticals, cosmetics and food preparation. Because this invention states not only the origin is mellow in mangrove forest endogenous fungus secondary metabolite gram Liewei may through the fermentation system, raw material is rich, moreover the extraction purification method is simple, suits the formalization production.

Description

technical field [0001] The present invention relates to a kind of crevetol, especially relate to a kind of endophytic fungus derived from mangroves - aspergillus dwarf clavatanicus BYY-1 (A.clavatonanicus) fungus metabolite crevetol as antioxidant in medicines, cosmetics and Uses in the food field. Background technique [0002] Free radicals with high reactivity will be continuously produced in the organism. Under normal conditions, these free radicals will be continuously removed by the antioxidant system, so that the free radicals in the body are in a dynamic balance. However, when the body's antioxidant defense system is damaged, or the body's metabolism is abnormal due to endogenous and / or exogenous stimuli, a large amount of free radicals will be produced. They can be toxic to the body, destroy biological macromolecules, affect cell activity, mainly damage cell membranes including vascular endothelial cell membranes and submicrostructures, and cause a series of oxidati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26A61K31/122A61P39/06C12R1/66
Inventor 郑忠辉黄珊珊沈月毛黄耀坚宋思扬杜希萍
Owner XIAMEN UNIV
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