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Albumen chip for detecting autoimmunity antibody of diabetes, as well as preparation and detection method

An autoimmune antibody and protein chip technology, applied in the field of biomedical research and clinical application, can solve the problems of difficult diagnosis and detection cost of diabetes clinical typing, avoid false positive and false negative detection results, and reduce detection cost , to avoid the effect of interference

Inactive Publication Date: 2004-10-27
XIAN BESTEDIT BIOMEDICAL SERVICES
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problems of difficult diagnosis and high detection cost of the existing clinical typing of diabetes

Method used

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  • Albumen chip for detecting autoimmunity antibody of diabetes, as well as preparation and detection method
  • Albumen chip for detecting autoimmunity antibody of diabetes, as well as preparation and detection method
  • Albumen chip for detecting autoimmunity antibody of diabetes, as well as preparation and detection method

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Embodiment

[0048] The invention relates to a protein detection chip, its preparation and detection method, and is mainly used for detecting three important indicators in the auxiliary diagnosis of diabetes: human glutamic acid decarboxylase autoantibody, human islet cell antigen 512 protein autoantibody and human insulin protein autoantibody . The present invention will be further described by the following examples, but it should not be understood that the scope of protection is limited to the following examples.

[0049] (1) A protein chip for detecting autoimmune antibodies in diabetes, comprising a housing 1 and a detection microarray 3, the housing 1 is a glass sheet with a specification of 22mm×75mm and a thickness of 1mm, and 78 pieces are arranged on the upper surface of the glass sheet Reaction pool 2, the reaction pool 2 is a square with a side length of 2.5 mm. A detection microarray 3 is arranged in each reaction pool 2, and the detection microarray 3 consists of three areas...

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Abstract

This invention relates to preparation and detect method of detection protein chip of diabetes autoimmune antibody. The scheme is as follows: a protein chip is a thin glass chip set with multiple reaction tanks on top surface of the said shell and a detection microarray in each of the reaction tank, every micro-array is composed of three different kinds, same size protein micro-solid phase detection chips of GAP, PTP and Insulin. The preparation method is to prepare protein micro-solid phase detection chips to be assembled and the detecting method is to apply indirect fluorescent method for detection.

Description

Technical field: [0001] The invention relates to the field of biomedical research and clinical application, in particular to a diabetes autoimmune antibody detection protein chip, and its preparation and detection methods. Background technique: [0002] Diabetes is currently one of the most serious diseases that endanger human health and quality of life. Its main symptom is a persistent increase in blood sugar concentration, and it endangers health through a variety of serious complications. According to the latest predictions made by the International Diabetes Federation (KDF) on the current situation of global diabetes and the World Health Organization (WHO) on the incidence of diabetes, 130 million people have been diagnosed with type II diabetes in the world, and more than 40 million people in my country . It is estimated that by 2025, the number of diabetic patients in the world will exceed 300 million, and the total number of diabetic patients in my country will be clo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N33/53G01N33/533G01N33/543G01N33/68
Inventor 刘镭罗毅王伟华
Owner XIAN BESTEDIT BIOMEDICAL SERVICES
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