Application of ampelopsin in preparation of medicine for preventing and treating AIDS

A technology of staphylosin and AIDS, which is applied in the field of application of staphylosin in the preparation of anti-AIDS drugs, and can solve the problems of inability to block the early infection process of HIV, expensive treatment, toxic and side effects, etc.

Inactive Publication Date: 2005-04-20
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-efficiency antiretroviral therapy has been used in recent years to improve the symptoms of AIDS patients after taking the drug, increase the number of CD4 cells, and reduce the viral load, but the treatment is expensive, and these drugs have the following disadvantages: (1) the ability to induce drug resistance HIV mutant strain; (2) short-term and long-term toxic side effects; (3) cannot block the early infection process of HIV
So far, there have been no reports on the application of staphylophyllin in the preparation of anti-AIDS drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the preparation of staphylophyllin.

[0017] The root of Cantonese snake grape, a plant of the genus Snakevine in the family Vitis genus, is used to prepare staphylophyllin by a conventional extraction method, and can be divided into powder injection ampoules of 10 mg each.

Embodiment 2

[0019] Take 10g of staphylophyllin and 10g of AZT, mix them evenly, and pack them into 1000 ampoules to make a compound preparation.

Embodiment 3

[0020] Example 3, antiviral (HIV) experiment of staphyloclin in vitro.

[0021] 1. Isolation and cell culture of human peripheral blood mononuclear cells (PBMCs)

[0022] Take whole blood from a healthy person anticoagulated with sodium citrate, dilute it with PBS, centrifuge at 1500r / min for 10 minutes, discard the plasma, suspend the blood cells, and slowly add it to the upper layer of the lymphocyte separation solution at a ratio of 2:1. Centrifuge at 2200r / min for 20min, suck out the PBMC layer between PBS and lymphocyte separation medium, wash twice with PBS, and finally wash with 10% FBS, 0.5% Hepes, PHA-P 10μg / ml, green chain double antibody 100U The RPMI 1640 complete medium was suspended, and the cell concentration was adjusted to 1×10 6 / ml in 5% CO 2 , cultured in a 37°C incubator for later use. MT-4 cell line and H9 cell line were cultured and passaged in RPMI 1640 complete medium containing 10% FBS, 0.5% Hepes, and 100 U of green chain double antibody.

[0023...

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PUM

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Abstract

Application of staphylophyllin in the preparation of anti-AIDS drugs. The present invention investigates the anti-HIV-1 effect of staphylosin in vitro, the regulation function of cellular immune function in the mouse immunosuppressive model, and observes the effect of staphylosin on human PBMC infected by HIV-1. S and MT-4 cell protection, and the inhibitory effect on HIV-1 co-receptor CXCR4, indicating that staphylosin has anti-HIV effect in vitro and can down-regulate PBMC S The CXCR4 on the surface can enhance the immune function of the mouse immunosuppressive model, indicating that staphylosin has the function of preventing and treating AIDS, and can be used to prepare medicaments for preventing and treating AIDS.

Description

technical field [0001] The invention relates to a medicine for preventing and treating AIDS, in particular to the application of staphylophyllin in the preparation of medicine for preventing and treating AIDS. Background technique [0002] AIDS, Acquired Immunodeficiency Syndrome (AIDS) is a disease caused by the Human Immunodeficiency Virus (HIV). Recently, the World Health Organization estimated that by the end of 2000, there had been a total of 57.9 million people infected with HIV and 21.8 million people who had died from AIDS. In 2000, 5.3 million people were newly infected with HIV and 3 million people died from AIDS. At present, it is estimated that 600,000 to 1,000,000 people have been infected in my country, and it has entered a period of rapid growth. AIDS is a social disease that involves a wide range of areas, and it is very difficult to prevent and control it. Although high-efficiency antiretroviral therapy has been used in recent years to improve the symptoms...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P31/18
Inventor 刘德育
Owner SUN YAT SEN UNIV
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