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Prepn process of corn stalk trichoderma as biological antiseptic

A technology of biological fungicide and corn stalks, which is applied in the direction of botany equipment and methods, fungicides, biocides, etc., can solve the problems of Trichoderma fungicide production cost increase, difficult to widely popularize and use, etc., to eliminate or reduce soil Antifungal effect, reducing incineration pollution, and increasing yield

Inactive Publication Date: 2007-01-17
田连生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the problem that exists at present is: preparation Trichoderma fungicide needs to use more expensive raw material such as corn flour, molasses, wheat bran, makes the production cost of Trichoderma fungicide increase, is difficult to popularize and use widely

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. Raw material processing: select non-mildew corn stalks, dry them in the sun and crush them into 20-mesh powder for later use;

[0018] b. liquid fermentation: medium composition: bran 0.5 gram, (NH 4 ) 2 SO 4 0.2 g, KH 2 PO 4 0.1 g, CaCO 3 0.1 grams, glucose 1.5 grams. 100 milliliters of liquid fermentation medium was prepared from the above-mentioned substances, the pH value of the liquid was adjusted to 5.5, and it was put into a 300 milliliter Erlenmeyer flask for autoclaving for 30 minutes. After the material was cooled, it was inserted into the screened antagonistic Trichoderma strain, shaken and cultured at 28°C for 40 hours, and the culture was stopped for later use.

[0019] c. Solid fermentation: take 9 grams of crushed corn stalk powder, (NH 4 ) 2 SO 4 0.12 g, MgSO 4 0.002 g, KH 2 PO 4 0.004 grams. Put the above-mentioned inorganic salt into a 300ml triangular flask and add 200ml of water to dissolve, then put in corn stalk powder, stir e...

Embodiment 2

[0022] a. Raw material processing: select non-mildew corn stalks, dry them in the sun and crush them into 20-mesh powder for later use;

[0023] b. Liquid fermentation: medium composition: 0.8 grams of bran, (NH 4 ) 2 SO 4 0.35 g, KH 2 PO 4 0.25 g, CaCO 3 0.2 grams, 1 gram of glucose. 100 milliliters of liquid fermentation medium was prepared from the above-mentioned substances, the pH value of the liquid was adjusted to 6, and the liquid was put into a 300 milliliter Erlenmeyer flask for autoclaving for 40 minutes. After the materials were cooled, they were inserted into the screened antagonistic Trichoderma strains, cultured with shaking at 29° C. for 42 hours, and the cultivation was stopped for later use.

[0024] c. Solid fermentation: Take 10 grams of crushed corn stalk powder, (NH 4 ) 2 SO 4 0.14 g, MgSO 4 0.004 g, KH 2 PO 4 0.045 grams. Put the above-mentioned inorganic salt into a 300ml triangular flask and add 200ml of water to dissolve, then put i...

Embodiment 3

[0027] a. Raw material processing: select non-mildew jade stalks, dry them in the sun and crush them into 20-mesh powder for later use;

[0028] b. Liquid fermentation: medium composition: 0.7 grams of bran, (NH 4 ) 2 SO 4 0.4 g, KH 2 PO 4 0.3 g, CaCO 30.3 grams, 2 grams of glucose. 100 milliliters of liquid fermentation medium were prepared from the above-mentioned substances, the pH value of the liquid was adjusted to 5.5, and put into a 300 milliliter Erlenmeyer flask for autoclaving for 35 minutes. After the material was cooled, it was inserted into the screened antagonistic Trichoderma strain, cultured with shaking at 30°C for 48 hours, and the culture was stopped for later use.

[0029] c. Solid fermentation: take 8 grams of crushed corn stalk powder, (NH 4 ) 2 SO 4 0.13 g, MgSO 4 0.003 g, KH 2 PO 4 0.005 grams. Put the above-mentioned inorganic salt into a 300ml triangular flask and add 200ml of water to dissolve, then put in corn stalk powder, stir ev...

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Abstract

The present invention is preparation process of corn stalk trichoderma as biological antiseptic and belongs to the field of agricultural antiseptic preparing technology. The corn stalk trichoderma as biological antiseptic is prepared with corn stalk as main material, and through material processing, liquid fermentation, solid fermentation, antiseptic preparation and other steps. During solid fermentation, ammonium sulfate, magnesium sulfate and potassium dihydrogen phosphate in certain proportion are added into corn stalk powder. The corn stalk trichoderma as biological antiseptic is used in preventing and treating fungal soil transmitted diseases of crop, and has high preventing and treating effect and excellent growth promoting and yield increasing effect, no environmental pollution and harm to human body and animal.

Description

technical field [0001] The invention relates to an agricultural biological fungicide, in particular to a method for preparing a trichoderma biological fungicide produced from corn stalks, and belongs to the technical field of agricultural microbial fungicides. Background technique [0002] In a country with large agricultural production, the straw after harvesting crops can be as high as hundreds of millions of tons each year, of which less than half are directly and indirectly returned to the field, another part is used as industrial raw materials or agricultural fuel, and a considerable part is burned. It not only wastes resources but also seriously pollutes the environment. Although various decrees prohibit the burning of stalks, they are repeatedly prohibited because of suffering from the lack of appropriate external management methods. On the other hand, because crops are often infested by various diseases and insect pests, resulting in reduced or even extinction of pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P3/00
Inventor 田连生李书生史延茂张根伟黄亚丽张丽萍董超程辉彩
Owner 田连生
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