Lung cancer transgene vaccine and its preparation method

A transgenic and vaccine technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, cells modified by introducing foreign genetic material, etc., can solve problems such as unsatisfactory effects

Inactive Publication Date: 2003-07-16
HANGZHOU FIRST PEOPLES HOSPITAL
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although people have spent a lot of energy, including surgery, radiotherapy, drug chemotherapy, immunization, tradit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lung cancer transgene vaccine and its preparation method
  • Lung cancer transgene vaccine and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. In this example, firstly, the cloned human IFN-γ full-length cDNA was introduced into human lung adenocarcinoma A by using retroviral vector PLXSN 548 In the cell line, G 418 Resistance screening resulted in a transformed cell line capable of secreting γ-interferon, which was expressed as A- 549 -IFN-γ.

[0018] Secondly, the vaccine is obtained by conventional cultivation of the cell line through the following steps:

[0019] 1) Logarithmic growth phase cells;

[0020] 2) pour out the culture solution;

[0021] 3) Washing: wash the cells once with 1640 serum-free medium;

[0022] 4) enzyme solution digestion;

[0023] 5) Blow and wash the cells with 1640 serum-free culture medium, centrifuge at 3500rpm×5min×2 times;

[0024] 6) counting;

[0025] 7) collecting cells;

[0026] 8) Add S180 antigen and immune adjuvant GM-CSF;

[0027] 9) Water bath: 37 degrees water bath for 30 minutes;

[0028] 10) Irradiation: Co 60 The tumor vaccine for injection...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A transgenic vaccine for lung cancer is prepared through targeting the cloned human IFN-gamma whole length CDMA to the cell strain A548 of human pulmonary adenocarcinoma by retroviral rector PLXSN, resistance screen to obatain cellstrain hu gamma-IFN-A549, and conventional culture to obtain the vaccine. It can prevent recurrence and transfer of tumor.

Description

technical field [0001] The invention relates to a lung cancer transgenic vaccine and a preparation method thereof. Background technique [0002] Lung cancer is a common disease that seriously endangers people's life and health, and is one of the most difficult malignant tumors to treat. At present, the morbidity and mortality of lung cancer at home and abroad are still rising. Lung cancer is the first among all malignant tumors in men and the second in women. Although people have spent a lot of energy, including surgery, radiotherapy, drug chemotherapy, immunization, traditional Chinese medicine, etc. to explore methods to prevent and treat lung cancer, the effect is still not satisfactory. The 5-year survival rate in foreign countries is only 13%. The latest domestic statistics show that the total 5-year survival rate of lung cancer in Shanghai is only 5.5%. In recent years, people have gradually realized that the main reason why the long-term surviva...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/00A61K45/00A61P35/00C12N5/10
Inventor 胡伟恩
Owner HANGZHOU FIRST PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap