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Lung cancer transgene vaccine and its preparation method

A transgenic and vaccine technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, cells modified by introducing foreign genetic material, etc., can solve problems such as unsatisfactory effects

Inactive Publication Date: 2003-07-16
HANGZHOU FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although people have spent a lot of energy, including surgery, radiotherapy, drug chemotherapy, immunization, traditional Chinese medicine, etc. to explore methods to prevent and treat lung cancer, the effect is still unsatisfactory

Method used

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  • Lung cancer transgene vaccine and its preparation method
  • Lung cancer transgene vaccine and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1. In this example, firstly, the cloned human IFN-γ full-length cDNA was introduced into human lung adenocarcinoma A by using retroviral vector PLXSN 548 In the cell line, G 418 Resistance screening resulted in a transformed cell line capable of secreting γ-interferon, which was expressed as A- 549 -IFN-γ.

[0018] Secondly, the vaccine is obtained by conventional cultivation of the cell line through the following steps:

[0019] 1) Logarithmic growth phase cells;

[0020] 2) pour out the culture solution;

[0021] 3) Washing: wash the cells once with 1640 serum-free medium;

[0022] 4) enzyme solution digestion;

[0023] 5) Blow and wash the cells with 1640 serum-free culture medium, centrifuge at 3500rpm×5min×2 times;

[0024] 6) counting;

[0025] 7) collecting cells;

[0026] 8) Add S180 antigen and immune adjuvant GM-CSF;

[0027] 9) Water bath: 37 degrees water bath for 30 minutes;

[0028] 10) Irradiation: Co 60 The tumor vaccine for injection...

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Abstract

A transgenic vaccine for lung cancer is prepared through targeting the cloned human IFN-gamma whole length CDMA to the cell strain A548 of human pulmonary adenocarcinoma by retroviral rector PLXSN, resistance screen to obatain cellstrain hu gamma-IFN-A549, and conventional culture to obtain the vaccine. It can prevent recurrence and transfer of tumor.

Description

technical field [0001] The invention relates to a lung cancer transgenic vaccine and a preparation method thereof. Background technique [0002] Lung cancer is a common disease that seriously endangers people's life and health, and is one of the most difficult malignant tumors to treat. At present, the morbidity and mortality of lung cancer at home and abroad are still rising. Lung cancer is the first among all malignant tumors in men and the second in women. Although people have spent a lot of energy, including surgery, radiotherapy, drug chemotherapy, immunization, traditional Chinese medicine, etc. to explore methods to prevent and treat lung cancer, the effect is still not satisfactory. The 5-year survival rate in foreign countries is only 13%. The latest domestic statistics show that the total 5-year survival rate of lung cancer in Shanghai is only 5.5%. In recent years, people have gradually realized that the main reason why the long-term surviva...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K45/00A61P35/00C12N5/10
Inventor 胡伟恩
Owner HANGZHOU FIRST PEOPLES HOSPITAL
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