Reforming composite insecticidal crystalline gene Cry2A of bacillus thuringiensis

A Bacillus thuringiensis gene technology, applied in the field of transformation and synthesis of Bacillus thuringiensis insecticidal crystal protein gene Cry2A, can solve the problems of incomplete transcripts, difficulty in detecting mRNA, mRNA instability, etc.
CN1480533AInactive Publication Date: 2004-03-10HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2004-03-10
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

A Bacillus thuringiensis insecticidal crystal protein DNA sequence Cry2A is designed and synthesized. Comparing it with original Cry2A DNA sequence can show that the amino acid components of DNa sequence coding protein are not changed, the application frequency of vegetable preference codon is higher and the AT sequence, reverse reduplicate sequence and indefinable subsequences are eliminated. It can be used for culturing the seed of insect-resisting transgenic plant.
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Description

Technical field:

[0001] The invention belongs to artificially transforming and synthesizing the DNA sequence of the Bacillus thuringiensis (Bt) insecticidal crystal protein (ICP) gene and a preparation method thereof. The DNA sequence of the present invention can be used as the technical field of molecular breeding for high-efficiency expression in transgenic insect-resistant plants. Background technique:

[0002] Insect infestation is an important factor causing loss of agricultural production. According to statistics, the direct economic loss caused by insect pests to agricultural production is as high as 13% every year.

[0003] Chemical pesticides have made important contributions to pest control and stable agricultural production. With people's awareness of the environmental hazards of chemical pesticides and the increasing awareness of environmental protection, environmentally safe biological pesticides have become a research hotspot. Am...

Claims

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