Transduction peptide-insecticidal crystal protein fased protein and its corresponding sequence and use

A technology of insecticidal crystal protein and fusion protein, applied in the field of genetic engineering, can solve the problems of loss of receptor protein, decreased sensitivity of target sites, loss of insecticidal efficacy, etc., and achieve high insecticidal activity
CN1683415AInactive Publication Date: 2005-10-19INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Publication Date
2005-10-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the field of gene engineering, relates to the constitution, expression and application of fusion protein, and is especially one kind of transduction peptide-insecticidal crystal protein fusing protein and its application. The PTD research results are applied in the field of microbial insecticidal crystal protein for the first time, so as to expand new field for applying PTD and open new way to eliminating pest's resistance. The fusion protein has pesticidal activity higher than that of single insecticidal crystal. The present invention has important theoretical and practical significance in controlling harmful organism and applying PTD.
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Description

【Technical field】

[0001] The invention belongs to the field of genetic engineering, and in particular relates to the construction of a fusion protein sequence and the expression and application of the fusion protein. The invention specifically provides a transduction peptide-insecticidal crystal protein fusion protein and application thereof. 【Background technique】

[0002] Transduction peptide (Protein Transduction Domain, PTD) (Schwarze SR, et al., Science, 1999, 285: 1569-1572) is a class that can automatically penetrate the cell membrane and can be used as a carrier to carry other biological macromolecules into the cell membrane An arginine-rich polypeptide. In general, the cell membrane of eukaryotic cells is impermeable to most proteins and polypeptides longer than 6 amino acids. In 1988, Green et al. (Cell.1988, 55:1179-1188) reported that the transactivator TAT from AIDS virus 1 (HIV-1) added to the cell culture medium could automatically enter the cultured cells t...

Claims

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