Method for raising stability for preserving catalase prepared by microbiological method

A catalase, storage stability technology, applied in the direction of enzyme stabilization, oxidoreductase, etc., can solve the problem of easy inactivation of liquid catalase, and achieve the effect of improving stability

Inactive Publication Date: 2005-01-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the stabilizer of the present invention can solve the problem of easy inactivation of liquid catalase, and significantly improve the stability of the enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The catalase prepared by liquid submerged fermentation was removed by suction filtration, and the enzyme activity was 2000U / mL. It was stored at 40°C for 30d and 60d, and the enzyme activity retention rates were 21.3% and 2.4%, respectively. After it was concentrated by ultrafiltration for 10 times, the enzyme activity was 20000U / mL, and stored at 40°C for 30d and 60d, the enzyme activity retention rates were 74.7% and 9.6%, respectively.

Embodiment 2

[0011] The stabilizer sodium chloride 20mmol / L was added to the ultrafiltered enzyme liquid, and it was stored at 40°C for 60 days, and the enzyme activity retention rate was 94.0%.

Embodiment 3

[0013] Add stabilizer gelatin 0.5g / L to the enzyme solution after ultrafiltration, store at 40°C for 60 days, and the enzyme activity retention rate is 93.9%.

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PUM

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Abstract

A method for increasing hydrogen peroxidase stability relates to a biological zyme used in clean production in textile industry. This invention takes the thermoascus aurantiacus IFO9862 as the initial strain to be prepared to get the hydrogen peroxidase after liquid deep fermentation to be ultrafitered, concentrated and added by certain stabilizers, NaCl, or glue or glycerol kept for 60days under 40deg.C its alive reservation is over 90%.

Description

technical field [0001] The invention relates to a method for improving the storage stability of catalase prepared by a microorganism method. It relates to a biological enzyme used in the clean production of the textile industry. Background technique [0002] Hydrogen peroxide is today the most commonly used bleaching agent for all natural fibers of vegetable or animal origin, and the process is ecologically acceptable and economically viable. Therefore, hydrogen peroxide bleaching or scouring "chlorine-free bleaching" process is becoming more and more widely used. However, the presence of hydrogen peroxide in the dyebath can cause fading of reactive dyes that are sensitive to oxidation. It has been shown that even small changes in dye molecules can lead to loss of color. Due to strict quality requirements, even slight color changes in dyed fabrics are not commercially acceptable. Therefore, once the bleaching process is over, in order to ensure the safety of subsequent d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/96
Inventor 陈坚王明星李寅华兆哲
Owner JIANGNAN UNIV
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