Method for preparing vindoline and Catharanthine
A technology of vinblastine and vindolin, which is applied in the field of natural medicine, can solve the problems such as the lack of comprehensive utilization of resources, and achieve the effect of low process cost.
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Embodiment 1
[0012] Take 10kg of the whole periwinkle plant, crush it and extract it three times by cold soaking with 20l 0.7% sulfuric acid, combine the extracts, adjust the pH to 7-8 with ammonia water, then use 10l chloroform for countercurrent continuous extraction, concentrate under reduced pressure, and then extract with a small amount of acetone Pine, get total weak alkaloid 20.5g. Dissolve the total weak alkaloids with 10ml of absolute ethanol to form a concentrated solution, then add 5% sulfuric acid ethanol solution to pH 3.8-4.1 and let stand overnight, crystallize out, and filter to obtain 1.5g of mixed vinblastine sulfate.
[0013] The filtrate was adjusted to pH 7-8 with aqueous ammonia, extracted with chloroform, and concentrated under reduced pressure to obtain 15 g of free alkaloid. The dry mixed sample was eluted with 50 g of macroporous adsorption resin (DA201) with ethanol: water (15:85, 30:70, 50:50, 70:30, 95:5), collected in 360 ml volume, and concentrated under redu...
Embodiment 2
[0015] Take 10kg of the whole periwinkle plant, crush it and extract it three times by cold soaking with 20l 0.7% sulfuric acid, combine the extracts, adjust the pH to 7-8 with ammonia water, then use 10l chloroform for countercurrent continuous extraction, concentrate under reduced pressure, and then extract with a small amount of acetone Pine, get total weak alkaloid 20.5g. Dissolve the total weak alkaloids with 10ml of absolute ethanol to form a concentrated solution, then add 5% sulfuric acid ethanol solution to pH 3.8-4.1 and let stand overnight, crystallize out, and filter to obtain 1.5g of mixed vinblastine sulfate.
[0016] The filtrate was adjusted to pH 7-8 with aqueous ammonia, extracted with chloroform, and concentrated under reduced pressure to obtain 15 g of free alkaloid. Dry mix the sample in 50g macroporous adsorption resin (HZ-841), elute with ethanol: water (15:85, 30:70, 50:50, 70:30, 95:5), collect in 50ml volume, depressurize concentrate. Vinblastine wa...
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