Method for preparing water-soluble quantum point of biological functionalized sugar

A biological functionalization and quantum dot technology, applied in the field of biochemistry and material science, can solve the problems of sugar recognition is only in the initial stage, limited function, complex structure, etc., and achieve the effect of low cost, easy operation and good repeatability

Inactive Publication Date: 2005-08-31
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] And because sugar is an indispensable part of life, it is closely related to more and more life phenomena, which has aroused the interest of scientists. However, the variety and complex structure of sugar make people's understanding of sugar difficult. It is still at a preliminary stage, therefore, there is an urgent need to understand the important role of sugars in the phenomenon of life, which requires the help of appropriate technology to understand its biological mechanism
However, at present, as a fluorescent marker with excellent performance, quantum dots are mostly limited to coupling with DNA, proteins, and small biological molecules. Quantum dots have not yet played a role in understanding carbohydrates.

Method used

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  • Method for preparing water-soluble quantum point of biological functionalized sugar
  • Method for preparing water-soluble quantum point of biological functionalized sugar
  • Method for preparing water-soluble quantum point of biological functionalized sugar

Examples

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Effect test

Embodiment 1

[0018] (1) Dissolving CdSe oil-soluble quantum dots in n-hexane to prepare a n-hexane solution of oil-soluble quantum dots with a concentration of 1mg / mL, CdSe oil-soluble quantum dots can adopt the method disclosed in Patent No. ZL 02 1 39152.1, but Prepared without the need for a sulfide stock solution.

[0019] (2) Take 0.5mL of the n-hexane solution of the above-mentioned oil-soluble quantum dots, add 3 times the volume of n-hexane to precipitate the oil-soluble quantum dots, and then redissolve the precipitate into a 0.5mL solution with n-hexane

[0020] (3) Follow the method of (2) for two more times, and finally dissolve it into a 2mL solution with n-hexane for use.

[0021] (4) Weigh 20 mg of carboxymethyl chitosan and put it in an agate mortar, add the above 2 mL quantum dot n-hexane solution, grind for 2-3 minutes, and place it in a fume hood until the n-hexane volatilizes completely.

[0022] (5) Add about 2mL of n-hexane into the mortar, grind for 2-3 minutes, and...

Embodiment 2

[0025] (1) CdSe / ZnS oil-soluble quantum dots are dissolved in n-hexane to prepare a n-hexane solution of oil-soluble quantum dots with a concentration of 1mg / mL. Method preparation.

[0026] (2) Take 0.5mL of the n-hexane solution of the above-mentioned oil-soluble quantum dots, add 3 times the volume of n-hexane to precipitate the oil-soluble quantum dots, and then redissolve the precipitate into a 0.5mL solution with n-hexane

[0027] (3) Follow the method of (2) for two more times, and finally dissolve it into a 2mL solution with n-hexane for use.

[0028] (4) Weigh 20 mg of carboxymethyl chitosan and put it in an agate mortar, add the above 2 mL quantum dot n-hexane solution, grind for 2-3 minutes, and place it in a fume hood until the n-hexane volatilizes completely.

[0029] (5) Add about 2mL of n-hexane into the mortar, grind for 2-3 minutes, and place it in a fume hood until the n-hexane is completely volatilized. Repeat this for 3-5 times.

[0030] (6) After the mor...

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Abstract

A process for preparing the water-soluble quantum site biofunctionalized by saccharide features that the saccharide with chelating biofunction is used as modifier and the quantum site is changed from oil-soluble one to water-soluble one by grinding white is biofunctionalized by saccharide.

Description

technical field [0001] The invention relates to a preparation method of sugar biofunctionalized water-soluble quantum dots, belonging to the fields of biochemistry and material science. Background technique [0002] In recent years, quantum dots, as a new type of inorganic nanomaterial, have played an important role in the fields of biological tracer labeling and cell imaging. Quantum dots have excellent fluorescence properties, strong fluorescence, narrow and symmetrical fluorescence emission peaks, controllable fluorescence color based on composition and size, continuous band excitation spectrum, and easy realization of unit excitation and multiple emission. However, at present, high-quality quantum dots are prepared by pyrolysis of organic phases, and the products are oil-soluble, while all biological systems are polar water-soluble. Quantum dots have application value only after biological functionalization. Therefore, modifying the surface of quantum dots by surface ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K51/04C09K11/00G01N33/533
Inventor 庞代文谢敏张志凌杜予民
Owner WUHAN UNIV
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