Functional fluorescent carbon dot as well as preparation method and application thereof

A fluorescent carbon dot and functional technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, chemical instruments and methods, etc., can solve the problems of high cost, radiation, unfavorable large-scale production and application of carbon dots, and achieve high safety , The effect of improving work efficiency

Inactive Publication Date: 2015-06-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Although the quantum yields of natural sugars, EDTA and TRIS are high, the synthetic materials are expensive, which is not conducive to the large-scale production and application of carbon dots
[0004] (2) Synthesis method issues
The synthesis time of the microwave method is short, but there is radiation in the synthesis process, which pollutes the environment and is not a green synthesis method.
These two synthetic methods are usually costly

Method used

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  • Functional fluorescent carbon dot as well as preparation method and application thereof
  • Functional fluorescent carbon dot as well as preparation method and application thereof
  • Functional fluorescent carbon dot as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Adopt coffee, cola as raw material.

[0026] Production of coffee carbon points Dissolve 2g of Nescafe original coffee in 20ml of hot water at 88°C, and centrifuge (14*10 3 rpm, 15min), remove the precipitate, get the supernatant, and filter. Through the gel column of Sephadex G-25, the carbon dots were separated and purified. The carbon dots with strong fluorescence intensity were collected by a fluorescence spectrophotometer. The collected carbon dots were vacuum freeze-dried.

[0027] Production of Coke Carbon Dots Put 100ml of Coca-Cola and Pepsi respectively on a magnetic stirrer and stir for 4 hours at a stirring speed of 500rpm / min to make the CO 2 The gas is released. The samples were passed through 0.45 μm and 0.22 μm filter membranes respectively, and put into a freeze dryer to freeze-dry. And the lyophilized sample was diluted to 20ml. Separation and purification by dextran G-25 column, excitation at 300 nm by a fluorescence spectrophotometer, and colle...

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Abstract

The invention discloses a carbon dot component in cola as well as a property characterization method and a practical application method of the carbon dot. The preparation method comprises the following steps: by taking original-taste (1+2) Nestle coffee, pepsi (330ml) and coca-cola (330ml) as main raw materials, separating and purifying through a dextrane gel G-25 column and performing freeze-drying for 24 hours to collect the carbon dot. The coffee/cola carbon dot is relatively low in cost, low in toxicity, simple in extracting method, good in water solubility, good in biocompatibility and can be widely used as a novel fluorescent carbon nano particle in the field of biomarkers.

Description

technical field [0001] The invention relates to fluorescent carbon nanometer materials, specifically fluorescent carbon nanoparticle existing in coffee and cola. Background technique [0002] Nanomaterials have good optical properties and have received more and more attention in the field of biotechnology. Among them, in many application fields such as biological detection, sensing, imaging, cytochemistry, immunohistochemistry, etc., the fluorescent labeling technology of nanomaterials has been well developed in recent years, and has become one of the most important technical means to realize the above functions. . However, the organic synthesis process of traditional fluorescent labeling materials is complicated, their biocompatibility is poor, and their solubility in water is very low, which limits their application in water phase to a certain extent. It is under this background that the research on fluorescent nanoparticles has gradually attracted the attention of schol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B31/02G01N21/64
Inventor 谭明乾姜程坤马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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