Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging
A technology of fluorescent carbon dots and cells, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of distinguishing and distinguishing liver cancer cells from other cancer cells, and achieves simple preparation, great application value, and fluorescence properties. excellent effect
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Embodiment 1
[0051] The preparation method of CDs includes the following steps:
[0052] 30 mg citric acid, 39 µL triethylenetetramine and 10.4 mg fluorescein (molar ratio = 1:1:5) were mixed and dissolved in 2 mL absolute ethanol, and 20 µLDMSO was added to prepare a precursor solution, which was placed in microwave reaction tube; place the microwave reaction tube in the microwave reactor and heat the precursor solution to 130 o C, reacted for 2 h; the obtained product was naturally cooled to room temperature; the obtained product was centrifuged at 6000 r / min to remove large particles; dialysis treated with a 1 KDa dialysis membrane for 72 h to obtain a purified CDs solution; The CDs solution was rotary evaporated to obtain CDs solid powder. The UV-Vis absorption spectrum, excitation spectrum and fluorescence emission spectrum of CDs solution are shown in the appendix. Figure 2-3 . Depend on figure 2 It can be seen that the carbon dots in this application absorb at 370 nm and 490 n...
Embodiment 2
[0054] The preparation method of CDs includes the following steps:
[0055] 30 mg of citric acid, 78 µL of triethylenetetramine and 4.16 mg of fluorescein (molar ratio = 1:2:2) were mixed and dissolved in 2 mL of absolute ethanol to prepare a precursor solution and placed in a microwave reaction tube ; Place the microwave reaction tube in a microwave reactor, heat the precursor solution to 120 °C, and react for 6 h; the obtained product is naturally cooled to room temperature; the obtained product is centrifuged at 6000 r / min to remove large particles; The purified CDs solution was obtained by dialysis treatment with a dialysis membrane with a specification of 1 KDa for 72 h; the CDs solution was rotary evaporated to obtain the CDs solid powder.
Embodiment 3
[0057] The preparation method of CDs includes the following steps:
[0058] 30 mg of citric acid, 195 µL of triethylenetetramine and 2.08 mg of fluorescein (molar ratio = 1:5:1) were prepared as a precursor solution and placed in a microwave reaction tube; the microwave reaction tube was placed in a microwave reaction tube In the reactor, the precursor solution was heated to 140 °C for 0.5 h; the obtained product was naturally cooled to room temperature; the obtained product was centrifuged at 6000 r / min to remove large particles; a dialysis membrane with a specification of 1 KDa was used After dialysis treatment for 72 h, purified CDs solution was obtained; CDs solution was rotary evaporated to obtain CDs solid powder.
[0059] Example of implementation effect
[0060] The application of CDs to the detection of cell viability in cells includes the following steps:
[0061] HeLa cells were seeded in confocal dishes at 37°C, 5% CO. 2 , cultured in a humidified cell incubator...
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