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Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging

A technology of fluorescent carbon dots and cells, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of distinguishing and distinguishing liver cancer cells from other cancer cells, and achieves simple preparation, great application value, and fluorescence properties. excellent effect

Active Publication Date: 2020-04-10
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention proposes a preparation method of fluorescent carbon dots and its application in cell imaging, which solves the technical problem that the prior art cannot distinguish liver cancer cells from other cancer cells by moving from lysosomes to nucleoli; and Through a fluorescent carbon dot, the technical problems of detecting cell activity, distinguishing normal cells and cancer cells, and distinguishing liver cancer cells among various cancer cells can be solved at the same time

Method used

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  • Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging
  • Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging
  • Preparation method of fluorescent carbon dots and application of fluorescent carbon dots in cell imaging

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Experimental program
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Effect test

Embodiment 1

[0051] The preparation method of CDs includes the following steps:

[0052] 30 mg citric acid, 39 µL triethylenetetramine and 10.4 mg fluorescein (molar ratio = 1:1:5) were mixed and dissolved in 2 mL absolute ethanol, and 20 µLDMSO was added to prepare a precursor solution, which was placed in microwave reaction tube; place the microwave reaction tube in the microwave reactor and heat the precursor solution to 130 o C, reacted for 2 h; the obtained product was naturally cooled to room temperature; the obtained product was centrifuged at 6000 r / min to remove large particles; dialysis treated with a 1 KDa dialysis membrane for 72 h to obtain a purified CDs solution; The CDs solution was rotary evaporated to obtain CDs solid powder. The UV-Vis absorption spectrum, excitation spectrum and fluorescence emission spectrum of CDs solution are shown in the appendix. Figure 2-3 . Depend on figure 2 It can be seen that the carbon dots in this application absorb at 370 nm and 490 n...

Embodiment 2

[0054] The preparation method of CDs includes the following steps:

[0055] 30 mg of citric acid, 78 µL of triethylenetetramine and 4.16 mg of fluorescein (molar ratio = 1:2:2) were mixed and dissolved in 2 mL of absolute ethanol to prepare a precursor solution and placed in a microwave reaction tube ; Place the microwave reaction tube in a microwave reactor, heat the precursor solution to 120 °C, and react for 6 h; the obtained product is naturally cooled to room temperature; the obtained product is centrifuged at 6000 r / min to remove large particles; The purified CDs solution was obtained by dialysis treatment with a dialysis membrane with a specification of 1 KDa for 72 h; the CDs solution was rotary evaporated to obtain the CDs solid powder.

Embodiment 3

[0057] The preparation method of CDs includes the following steps:

[0058] 30 mg of citric acid, 195 µL of triethylenetetramine and 2.08 mg of fluorescein (molar ratio = 1:5:1) were prepared as a precursor solution and placed in a microwave reaction tube; the microwave reaction tube was placed in a microwave reaction tube In the reactor, the precursor solution was heated to 140 °C for 0.5 h; the obtained product was naturally cooled to room temperature; the obtained product was centrifuged at 6000 r / min to remove large particles; a dialysis membrane with a specification of 1 KDa was used After dialysis treatment for 72 h, purified CDs solution was obtained; CDs solution was rotary evaporated to obtain CDs solid powder.

[0059] Example of implementation effect

[0060] The application of CDs to the detection of cell viability in cells includes the following steps:

[0061] HeLa cells were seeded in confocal dishes at 37°C, 5% CO. 2 , cultured in a humidified cell incubator...

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Abstract

The invention belongs to the field of nano material manufacturing, and relates to a method for preparing carbon dots by using a microwave-assisted method and application, in particular to a preparation method of fluorescent carbon dots and application of the fluorescent carbon dots in cell imaging. The cell activity can be detected, normal cells can be distinguished from cancer cells and cell membranes can be targeted specific to liver cancer cells. According to the method, citric acid, triethylenetetramine and fluorescein are used as raw materials, ethyl alcohol is used as a solvent, DMSO isused as a cosolvent, a reaction is performed for 2-8 hours at the temperature of 100-140 DEG C by utilizing the microwave-assisted method, and the fluorescent carbon dots are obtained through dialysisand rotary evaporation. The method is easy to operate, easily available in raw materials, green and environment-friendly. The carbon dots prepared by the method can be simultaneously used for detecting cell activity in cells, distinguishing normal cells from cancer cells and targeting cell membranes specific to liver cancer cells. The potential application value is realized in the fields of biology, medical treatment and the like.

Description

technical field [0001] The invention belongs to the field of nanomaterial manufacturing, and relates to a method for preparing carbon dots by a microwave-assisted method and its application in cell imaging, in particular to a method for preparing fluorescent carbon dots and its application in cell imaging. The fluorescent carbon dots set can be used to detect cell activity, distinguish normal cells from cancer cells, and specifically distinguish liver cancer cells in various cancer cells. Background technique [0002] After long-term research, carbon dots (CDs), as a new type of nanomaterials with small particle size, have been widely used in fluorescence sensing, biomarkers, cancer diagnosis, solar cells, etc. due to their unique optoelectronic properties. Applications. Compared with traditional semiconductor quantum dots (QDs) and organic dyes, carbon dots have a wide range of raw materials, environmental protection, good water solubility and chemical inertness, strong re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/65G01N21/64B82Y20/00B82Y30/00
CPCC09K11/65C09K11/02G01N21/6428G01N21/6456B82Y20/00B82Y30/00
Inventor 李朝辉郭逸菲孙远强屈凌波
Owner ZHENGZHOU UNIV
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