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PTD-SOD fusion protein and application of its derivative in cosmetics

A technology of fusion proteins and derivatives, applied in the field of fusion proteins, can solve the problems of inability to form intracellular vesicles with exogenous proteins, low efficiency, cell death, etc. effects of aging

Inactive Publication Date: 2005-10-26
WUHAN YANSEN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-viral carrier methods include: microinjection, electroporation, liposomes, endocytosis mediated by bacterial toxins, red blood cells or other receptors, etc.; Die or form intracellular vesicles that prevent foreign proteins from efficiently transmembrane into the cell [2]

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] 1. Preparation of superoxide dismutase face cream:

[0023] 1.1 Preparation of fusion protein (TAT-SOD) of recombinant TAT protein transduction domain (TAT-PTD) and human copper, zinc SOD (hCu, Zn SOD):

[0024] The hCu, Zn SOD cDNA derived from human hepatocytes was cloned by RT-PCR, and then PCR was amplified with primer A containing the coding gene of TAT-PTD at the 5' end and primer 2 complementary to the 3' end of hCu, Zn SOD cDNA. Add hCu, Zn SOD and TAT-PTD fusion genes. After the amplified product was recovered by enzyme digestion and electrophoresis, it was inserted into the multiple cloning site of plasmid pET21, and the expression plasmid pET-TAT-SOD was constructed, transformed into Escherichia coli BL21(DE3), and positive clones were screened for identification by enzyme digestion.

[0025]Pick a positive single colony and inoculate it in the LB medium containing ampicillin, grow for about 2 hours at 37°C, OD 600 When it reaches about 0.6, add IPTG to a f...

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Abstract

An application of PTD-SOD fusion protein and its derivatives in preparing the cosmetics for whitening and beautifying skin, treating acne, scar, wrinkles, etc, preventing pigmentation and senile plaque, and delaying skin senility is disclosed.

Description

technical field [0001] The invention relates to the field of fusion proteins, in particular to the application of protein transduction domain (Protein Transduction Domain, PTD)-linked superoxide dismutase (Superoxide Dismutase, SOD) fusion protein and its derivatives in cosmetics. Background technique [0002] As we all know, due to the ultraviolet rays in the sun and many other factors in life, such as computer radiation, smoking and drinking, stress, junk food, etc., will increase the production of free radicals in the body, accelerate the oxidation of cells, and lead to accelerated aging of the skin, especially It is ultraviolet rays, after it penetrates into the skin, it will produce harmful free radicals (Free Radicals), these free radicals will increase the number of epithelial lipid peroxides (LPO), destroy the collagen and elastic fibers of the skin, and also Stimulate melanocytes and increase the secretion of melanin [1] , resulting in dry and loose skin, loss of e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
Inventor 饶平凡刘树滔
Owner WUHAN YANSEN TECH
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