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Polymerase chain reaction biochip temperature control method

A temperature control method and biochip technology, applied in the direction of fermentation, etc., can solve the problems that the influence of saturation cannot be completely solved, and there is no practical application of PCR biochip temperature control system, etc., to achieve excellent steady-state temperature control characteristics and good dynamic characteristics , the effect of improving efficiency

Inactive Publication Date: 2005-12-21
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The incremental PID method can resist overshooting and improve dynamic performance to a certain extent, but it still cannot completely solve the effect of saturation, so it has not been practically applied to the temperature control system of PCR biochips

Method used

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  • Polymerase chain reaction biochip temperature control method
  • Polymerase chain reaction biochip temperature control method

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Embodiment Construction

[0039] Such as figure 1 Shown is the internal structure diagram of the fuzzy logic controller. The complete fuzzy logic controller is composed of fuzzer, fuzzy inference engine and defuzzifier. The input to the controller, in this invention the error e, is an exact value. The fuzzer fuzzifies this precise input error e into human language zero, small, and large, corresponding to the fuzzification of input parameters in building a fuzzy logic controller. According to the input value, through the fuzzy reasoning machine, the novel fuzzy reasoning rule similar to Table 1 is stored in the fuzzy reasoning machine of the present invention, adopts certain reasoning mechanism, obtains different fuzzy output, corresponds to constructing the fuzzy logic controller in Rules for Fuzzy Reasoning. After the fuzzy output passes through the defuzzifier, the precise output quantity is obtained, and the outputs of the two fuzzy controllers are respectively k p and k i , this process corresp...

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Abstract

The polyase chain-reaction biochip temperature control method includes: Set up integrating factor, when the error is greater, make the controller reach output saturation in a short time, the temperature rises or drops at full speed, relatively weaker controller saturation degree has guaranteed very fast speed of desaturation. When the error is less, uses ordinary integrating function to dispel the stable state error after the desaturation. Set up suitable factor of proportionality, enter the false integrating saturated condition fast with the auxiliary controller when the error is greater. Use the integrating function to resist the exceed adjusting when the error is less, uses ordinary proportion function to output steadily after the desaturation.

Description

technical field [0001] The invention relates to a control method of a biological detection chip, in particular to a temperature control method of a polymerase chain reaction biological chip. Used in the field of microelectromechanical systems. Background technique [0002] Polymerase chain reaction, or PCR (Polymerase Chain Reaction, PCR), is a primer-mediated enzymatic synthesis of DNA sequences in vitro, and is a reaction at three different temperatures consisting of temperature control and enzyme-catalyzed processes. Periodically repeat the process. Applying different temperatures will produce different biological reactions, among which 93°C is the DNA denaturation temperature, 50°C is the annealing temperature, and 74°C is the extension temperature. Repeating these three temperature ranges can achieve DNA amplification. The conventional PCR method has disadvantages such as large heat capacity, slow heating and cooling speed, and expensive biochemical reagent consumptio...

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Application Information

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IPC IPC(8): C12P19/34
Inventor 陈文元邵诗逸牛志强张卫平
Owner SHANGHAI JIAO TONG UNIV
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