Mucor for producing picropodophyllin and its glucoside and kaempferol flavone kind substance, its method and application

A technology of kaempferol flavonoids and podophyllotoxin, which is applied in the field of microorganisms, can solve problems such as increased demand, destruction of plant resources, loss of biodiversity and ecological environment, etc., achieves short production cycle, simple nutritional requirements, and protection of plant ecology The effect of diversity

Inactive Publication Date: 2006-01-11
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the broad application prospects of podophyllum substances in medicine and agriculture and forestry control, their demand is increasing day by day. The massive collection of this kind of plant raw materials has caused serious damage to this kind of plant resources; causing biodiversity and ecological environment irreparable loss

Method used

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  • Mucor for producing picropodophyllin and its glucoside and kaempferol flavone kind substance, its method and application
  • Mucor for producing picropodophyllin and its glucoside and kaempferol flavone kind substance, its method and application
  • Mucor for producing picropodophyllin and its glucoside and kaempferol flavone kind substance, its method and application

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Experimental program
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Effect test

Embodiment 1

[0044] The Taoerqi plant was collected from Taibai Mountain, and the pure strain GJ-TW8 was isolated, purified and screened out by tissue separation method and preserved for future use.

[0045] PDA medium: 100ml of 20% potato juice, 2g of sucrose, pH 6.5. Add 2g of agar to the seed medium; the fermentation medium is liquid.

[0046]The GJ-TW8 bacterial strain provided by the present invention is connected to the seed medium, cultivated for 5 days at 28°C, transferred to a 500ml Erlenmeyer flask with 200ml of fermentation medium, 29°C, 180r / min, and shakes the flask for 7 days. Collect 40L of the fermentation culture, filter with suction, collect about 4250g of wet mycelium, take it out after freezing for 4 hours, break the mycelium, and extract the broken mycelium with equal volumes of chloroform and n-butanol in turn. The chloroform extract and the n-butanol extract were collected respectively, and concentrated under reduced pressure at 60°C to 10 ml.

[0047] The concentr...

Embodiment 2

[0050] ISP fermentation medium: soluble starch 10g, K 2 HPO 4 1.0g, MgSO 4 1.0g, NaCl 1.0g, (NH 4 ) 2 SO 4 2.0g, trace element solution 1ml, distilled water 1000ml, pH6.8 (trace element solution: FeSO 4 ·7H 2 O 0.1g, MnCl 2 4H 2 O 0.1g, ZnSO 4 ·7H 2 O 0.1g, distilled water 100ml)

[0051] The GJ-TW8 bacterial strain provided by the present invention is inoculated in PDA seed culture medium 28 ℃, after cultivating 5 days, transfers in the 500ml Erlenmeyer flask that 200ml ISP fermentation medium is housed, 29 ℃, 180r / min, shaking flask culture 7 days, Collect 3160 g of wet mycelium, freeze for 4 hours, break the mycelium, and extract the broken mycelium with equal volumes of chloroform and n-butanol respectively. The chloroform extract and the n-butanol extract were collected respectively, and concentrated under reduced pressure at 60°C to 10 ml.

[0052] The concentrated chloroform extract was separated by silica gel column chromatography (silica gel 200-300 mes...

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Abstract

A mucor podophyllus GJ-TW8 (CCTCC No. M205032) used to prepare podophyllotoxin, its glucoside and kaemplerol flavone is prepared from the root of sinopodophyllum through separation, screening and purifying. It has multiple medicine values.

Description

technical field [0001] The invention relates to a new mucormycete and its method and application for producing podophyllotoxin and its glycosides and kaempferol flavonoids, belonging to the technical field of microorganisms. Background technique [0002] Podophyllotoxin (PPT) and kaempferol flavonoids are a class of bioactive substances with medicinal value that exist in the Berberidaceae (Berheridaceae), that is, podophyllum plants. In 1880, Podwyssotzkii isolated podophyllotoxin crystals for the first time from acanthus resin. In the 1940s, King et al. reported that podophyllotoxin has a colchicine-like effect and can cause cell mutation. A series of medical and biological studies later confirmed that podophyllotoxin has anti-tumor activity. However, due to its high toxicity, its clinical application is limited. In the 1950s, the relationship between its activity and structure-activity was studied in depth, and a large number of derivatives based on it were synthesized a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12P7/26C12P19/00
Inventor 黄建新
Owner NORTHWEST UNIV(CN)
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