Eu-labelling early diagnosis kit for cytomegalovirus infection

A cytomegalovirus and early diagnosis technology, which is applied in the field of active infection of cytomegaloocytes, can solve problems such as low sensitivity and poor stability, and achieve the effects of improving sensitivity, good stability and easy preparation.

Inactive Publication Date: 2006-02-08
ZHEJIANG UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of low sensitivity and poor stability in the prior art, adopt europium labeling technology, and provide an early diagnosis kit for europium-labeled cytomegalovirus infection, so as to improve the sensitivity and stability of the method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Eu-labelling early diagnosis kit for cytomegalovirus infection
  • Eu-labelling early diagnosis kit for cytomegalovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Referring to Fig. 1, the present invention consists of a box body 1, a coated plate 2, a liner 3, a europium-labeled antibody 4, a negative quality control product 5, a positive quality control product 6, a dilution buffer 7, a reaction buffer 8, a washing buffer Liquid 9, enhancement liquid 10, and operating instructions 11, the liner 3 is provided with a container hole, respectively placed europium-labeled antibody 4, negative quality control product 5, positive quality control product 6, buffer for dilution 7, and reaction buffer 8 , wash buffer 9, enhancement solution 10.

[0015] Reagent composition: plate coated with specific CMV recombinant antigen, Tris reaction buffer (50mmol / LTris-HCl, containing 0.9% NaCl, 0.5% BSA solution), buffer for sample dilution, europium-labeled anti-IgM antibody (~0.2 mg / ml), washing solution for plate washing, fluorescence enhancement solution, negative and positive quality control products.

[0016] When in use, after returning th...

Embodiment 2

[0018] positive case

[0019] The results show that the kit of the invention has high sensitivity and can detect infection earlier.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an enropin label idioldast viral infection early stage quotation which is formed by a box 1, a uterus plate 2, a pad 3, an enropin label antibody 4, a negative nature control object 6, a dilution buffer liquid 7, a reacting buffer liquid 8, a washing buffer liquid 9, a reinforcing liquid 10, and an operating instruction 11.

Description

technical field [0001] The invention relates to a detection reagent for indicating the early infection of cytomegalovirus, which can identify the active infection state of cytomegalovirus. Background technique [0002] At present, enzyme-linked immunosorbent assay reagents are mostly used in the market to determine cytomegalovirus infection. When the human body is infected by cytomegalovirus, IgM antibodies are first produced, and the antibody presents a concentration curve that first increases and then decreases in the body. Enzyme labeling technology mostly uses horseradish peroxidase, which is easily affected by ambient temperature and pH value, and the enzyme itself is a macromolecular protein, which has a space-occupying effect on the labeled protein, resulting in low sensitivity and poor stability of the method. Contents of the invention [0003] The purpose of the present invention is to overcome the disadvantages of low sensitivity and poor stability in the prior a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
Inventor 尚世强赵正言俞锡林
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap