Determination method of proangiotension transferase activity and proangiotension transferase diagnosis kit

A technology of angiotensin and diagnostic kits, which is applied in the field of medical testing and measurement, can solve the problems of unpopularity, achieve high precision, accurate test results, and facilitate popularization and application

Inactive Publication Date: 2006-06-14
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The methods for measuring ACE activity in serum include: biological method, colorimetric method, radioisotope method, fluorescence photometry, high pressure liquid chromatography and immunoassay, among which the sensitivity and precision of the latter four methods are better, but Due to the constraints of instruments and reagents, it has not been popularized

Method used

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  • Determination method of proangiotension transferase activity and proangiotension transferase diagnosis kit
  • Determination method of proangiotension transferase activity and proangiotension transferase diagnosis kit
  • Determination method of proangiotension transferase activity and proangiotension transferase diagnosis kit

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Experimental program
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Effect test

Embodiment 1

[0031] Prepare an angiotensin-converting enzyme activity diagnostic kit according to the following ingredients and dosage:

[0032] Tris-HCl buffer 80mmol / l,

[0033] FAPGG 0.3mmol / l,

[0034] Ethylene glycol 20mmol / l.

[0035] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the tested angiotensin-converting enzyme sample to the reagent is 1 : 25, the reaction direction is negative reaction (absorbance decreases, the same below), the delay time is 1 minute, the detection time is 2 minutes, and the theoretical K value is -52000.

[0036] Add the sample and the prepared reagent, and the two are automatically mixed inside the analyzer to detect and record the decrease of the absorbance at 340nm. According to methods such as rate method and end point method, the activity of angiotensin-converting enzyme in the sample is me...

Embodiment 2

[0038] Prepare an angiotensin-converting enzyme activity diagnostic kit according to the following ingredients and dosage:

[0039] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0040] FAPGG 1.2mmol / l,

[0041] Glycerol 25mmol / l.

[0042] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the measured angiotensin converting enzyme sample to the reagent is 1 : 25, the reaction direction is negative reaction, the delay time is 1 minute, the detection time is 2 minutes, and the theoretical K value is -52000.

[0043]Add the sample and the prepared reagent, and the two are automatically mixed inside the analyzer to detect and record the decrease of the absorbance at 340nm. According to methods such as rate method and end point method, the activity of angiotensin-converting enzyme in the sample is measured and calculated b...

Embodiment 3

[0045] Prepare an angiotensin-converting enzyme activity diagnostic kit according to the following ingredients and dosage:

[0046] Triethanolamine buffer 120mmol / l,

[0047] FAPGG 2mmol / l,

[0048] Propylene glycol 30mmol / l.

[0049] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test secondary wavelength 405nm or more, the volume ratio of the measured angiotensin converting enzyme sample to the reagent is 1 : 25, the reaction direction is negative reaction, the delay time is 1 minute, the detection time is 2 minutes, and the theoretical K value is -52000.

[0050] Add the sample and the prepared reagent, the two are automatically mixed inside the analyzer, and the following principle reactions occur:

[0051]

[0052] Detect and record the decrease of absorbance at 340nm. According to methods such as rate method and end point method, the activity of angiotensin-conver...

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Abstract

The invention relates to angiotensin converting enzyme activity measuring method and its diagnosis kit. It is applied artificial synthesis substrate FAPGG to directly react with angiotensin converting enzyme to form furyl-acryloyl-phenylalanine, and to measure its activity by 340nm absorbance lowering speed. The method has high specificity, good accuracy. And it can be quickly measured by ultraviolet/visible light analyzer or semi/full automatic biochemical analysis. Its measuring cost is low; and it is convenient for generalization and application.

Description

technical field [0001] The invention relates to a method for measuring the activity of angiotensin-converting enzyme by enzymatic method, and an angiotensin-converting enzyme diagnostic kit prepared by using the method, belonging to the technical field of medical examination and measurement. Background technique [0002] The main physiological function of angiotensin-converting enzyme (ACE for short) is to convert angiotensin I into angiotensin II. The methods for measuring ACE activity in serum include: biological method, colorimetric method, radioisotope method, fluorescence photometry, high pressure liquid chromatography and immunoassay, among which the sensitivity and precision of the latter four methods are better, but Due to the constraints of instruments and reagents, it has not been popularized. Spectrophotometry is currently used more, and it has a variety of determination methods. [0003] The reaction principle of measuring ACE activity by ultraviolet spectropho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/25
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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