Reagent system and method for testing activity of phenylalnine ammonialyase
A technology for phenylalanine ammonia lyase and activity determination, which is applied in biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems affecting the accuracy of determination results, interference, etc., and achieves extended and accurate fluorescence retention time. Active and sensitive effects
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Embodiment 1
[0074] Embodiment 1 is the mensuration of jujube phenylalanine ammonia-lyase activity, and the molar concentration of assay reagent composition is as follows:
[0075] Buffer: 100mmol / L, pH 8.8 tris-hydrochloric acid solution;
[0076] Reagent 1: L-phenylalanine 30mmol / L, prepared with the above buffer as a solvent;
[0077] Reagent 2: succinic acid 150mmol / L, prepared with the above buffer as a solvent;
[0078] Reagent 3: Glycyl-L-leucine 5mmol / L, prepared with the above buffer as a solvent;
[0079] Reagent 4: ninhydrin 50mmol / L, prepared with the above buffer as a solvent;
[0080] Stop solution 1: hydrochloric acid 6.0mol / L, prepared with the above buffer as a solvent;
[0081] Stop solution 2: Potassium sodium tartrate 10mmol / L, sodium carbonate 200mmol / L, copper sulfate 20mmol / L, prepared with the above buffer as a solvent;
[0082] Calibration solution: 20mmol / L L-phenylalanine standard solution, prepared with the above buffer as a solvent.
[0083] Embodiment 1 a...
Embodiment 2
[0102] Embodiment 2 is the mensuration of Malin jujube phenylalanine ammonia solution activity, and the molar concentration of assay reagent composition is as follows:
[0103] Buffer: 10mmol / L, pH 9.0 tris-hydrochloric acid solution;
[0104] Reagent 1: L-phenylalanine 10mmol / L, prepared with the above buffer as a solvent;
[0105] Reagent 2: succinic acid 200mmol / L, prepared with the above buffer as a solvent;
[0106]Reagent 3: Glycyl-L-leucine 50mmol / L, prepared with the above buffer as a solvent;
[0107] Reagent 4: ninhydrin 100mmol / L, prepared with the above buffer as a solvent;
[0108] Stop solution 1: hydrochloric acid 6.0mol / L, prepared with the above buffer as a solvent;
[0109] Stop solution 2: Potassium sodium tartrate 100mmol / L, sodium carbonate 500mmol / L, copper sulfate 100mmol / L, prepared with the above buffer as a solvent;
[0110] Calibration solution: 20mmol / L L-phenylalanine standard solution, prepared with the above buffer as a solvent.
[0111] Emb...
Embodiment 3
[0130] Embodiment 3 is the mensuration of plum fruit phenylalanine ammonia-lyase activity, and the molar concentration of assay reagent composition is as follows:
[0131] Buffer: 200mmol / L, pH 8.0 tris-hydrochloric acid solution;
[0132] Reagent 1: L-phenylalanine 200mmol / L, prepared with the above buffer as a solvent;
[0133] Reagent 2: succinic acid 200mmol / L, prepared with the above buffer as a solvent;
[0134] Reagent 3: Glycyl-L-leucine 10mmol / L, prepared with the above buffer as a solvent;
[0135] Reagent 4: ninhydrin 30mmol / L, prepared with the above buffer as a solvent;
[0136] Stop solution 1: hydrochloric acid 6.0mol / L, prepared with the above buffer as a solvent;
[0137] Stop solution 2 (basic copper solution): Potassium sodium tartrate 1.0mmol / L, sodium carbonate 100mmol / L, copper sulfate 1.0mmol / L, prepared with the above buffer as a solvent;
[0138] Calibration solution: 20mmol / L L-phenylalanine standard solution, prepared with the above buffer as a s...
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