Tissue culture medium and fast propagation method for Sorbone lily

A technique for tissue culture and culture medium of lily, which is applied in the biological field and can solve problems such as inability to scale explants

Inactive Publication Date: 2006-08-02
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above medium and method cannot be used as explants for the scales of the bulb of Sorbonne (pink)

Method used

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Experimental program
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Effect test

Embodiment 1

[0025] 1 Materials and methods: as described in 1 and 2 in the above specific embodiment

[0026] 2 Results: 2.1 Induction of adventitious buds of lily scales

[0027] The scales were inserted into the medium and cultured in the tissue culture room for 7 days in the dark. The color of the scales changed from white to green in about 15 days. White and light green protrusions appeared on the surface of the scales and at the incision at 20 days. There are several such protrusions on each scale. At 40-45d, dense green bud clusters grew on the protrusions, and at the same time, the calluses at the base of the scales increased, and at 70-80d, the bud clusters that had emerged continued to grow taller, while the callus at the base grew. New buds and bud clusters appear. By 60d (approximately 8 weeks), the differentiation rates of buds in the four induction media were all greater than 40%. See Table 1 for induction culture conditions. The normal growth of the bud cluster on the ind...

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Abstract

The present invention belongs to the field of biotechnology, and is tissue culture method to propagating great amount of bacteria-free Sorbone lily seedlings. Sorbone lily scale as explant, after sterilization and cutting into blocks, is inoculated on culture medium with different plant hormones to induce callus and cespitose bud; callus and cespitose bud are proliferated in secondary culture medium until reaching seedling height of 5 cm and forming base bulb; and the seedling is transplanted in rooting culture medium to harden off for seven days before cultivating in culturing medium. The inducing culture medium consists of MS+2ú¼4-D 1.0í½2.0mg / L and MS+BA1.5 mg / L+NAA 0.2 mg / Lú”the proliferation culture medium includes MS+2ú¼4-D 1.0í½1.5mg / L and MS+BA 0.5mg / L+NAA 0.2 mg / Lú”and the rooting culture medium includes 1 / 2MS+IBA 0.2mg / L and NAA 0.2mg / L. The culture conditions include temperature of 22í½26 deg.c, illuminance of 2000í½2200lx, lighting 12 hr / day and pH 5.8.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly propagating lily tissue culture seedlings, which comprises selecting parts of explants of Lilium solbonn, induction, proliferation and subculture, formula of rooting medium, culture time, conditions and transplantation of tissue cultured seedlings. Planting conditions. Background technique [0002] Lilium tenuifolium Fisch is a general term for plants of the genus Lilium in the family Liliaceae. Lilium is a world-renowned ornamental flower with medicinal and edible value. The Sorbonne lily (Sorbonne) belongs to The Oriental Hybrids. It is a rare variety group among lilies. One of the most famous flower species, Oriental Lily is bred through cross breeding, and the seeds are highly aborted. Traditional bulb bulb propagation methods are often used, and the propagation speed is slow. It is difficult to meet market demand in the short term. At present, domestic bulbs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/00C12N5/04
Inventor 陈建群张伟丽金欣庆袁慧中经志强
Owner NANJING UNIV
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