Method for regenerating bitter herbs corm by in-vitro culture

A technology of in vitro culture and bitter grass balls, applied in the field of plant tissue culture, can solve the problems of difficult large-scale propagation, large impact of winter buds, low settlement survival rate, etc., and achieve the effect of curbing browning and pollution

Inactive Publication Date: 2006-09-06
NANJING UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seedlings of this plant have a low settlement survival rate under natural conditions, and the germination of winter buds is greatly affected by the thickness of the soil layer. A large number of living winter buds in the soil layer cannot germinate into bitter grass plants, and it is difficult to propagate on a large scale.
[0004] At present, there is no relevant report on the research on the tissue culture technology of E.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The method is carried out in a plant tissue culture room, and three batches of approximately 12,000 engineering seedlings of Erythrina fragrans have been cultivated successively. The specific method is: on the basis of MS basic medium, the callus induction culture is carried out by using the bulbs of the bitter grass, and the callus is induced by the bulbs of the bitter grass under the condition of adding 2,4-D hormone, and the callus induction frequency reaches More than 80%. Under the condition of adding BA hormone, the regenerated plants were induced and cultured; the regeneration frequency of the plants was 90%. Under the condition of adding NAA and IBA hormones, complete rooted regenerated plants are cultivated; the rooting rate of test-tube plantlets reaches 100%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
induction rateaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of plant tissue culture technique. The invention employs the regenerative method of isolated culture of kuh-weed bulb to produce high grade tissue sprout of kuh-weed in large scale. The method overcomes the shortage of low survival rate in natural condition of the seed, affective to soil thickness of germination in winter and non germination of a large amount of winter sprout to grow into plant. The invention can meet demand of establishing aquatic vegetation and reduce water pollution.

Description

1. Technical field [0001] The invention belongs to the technical field of plant tissue culture. 2. Background technology [0002] The pollution of my country's water ecosystem is serious, and the decline of water environment quality has caused serious damage to aquatic biological resources. It is of great significance to restore the balance of aquatic ecosystem, purify water quality, promote the development of fishery, and introduce water purification plants. [0003] Bitter grass is a submerged herbaceous plant of the genus Vallisneria in the Hydrocharitaceae family Hydrocharitaceae. Bitter grass is rich in nutrients and complete in ingredients. It is an important bait for herbivorous fish and benthos; winter buds of bitter grass are important food for some wild rare birds during winter; Erythrina fragrans has wide ecological adaptability and strong ability to absorb dirt. It is an important submerged plant for reducing water pollution and eutrophication. Its recovery time ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C02F3/32
Inventor 安树青郑丽屏周长芳关保华蒋金辉
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap