Human angioma rat animal model and its configuration method

A technology of animal models and construction methods, applied in teaching models, educational tools, instruments, etc., can solve the problems of large differences in hemangiomas

Active Publication Date: 2006-10-25
SUZHOU BOJUHUA BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are two defects in this model: 1. The hemangioendothelioma cells used in the model are malignant tumors, which are obviously different from benign hemangioma cells, so the established animal model is quite different from hemangioma; 2. .The hemangioendothelioma cells are derived from mice rather than humans, so it is not very ideal to use it to study the mechanism of human hemangioma or to screen effective drugs for human hemangioma

Method used

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  • Human angioma rat animal model and its configuration method
  • Human angioma rat animal model and its configuration method
  • Human angioma rat animal model and its configuration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Isolation and culture of human hemangioma endothelial cells

[0033] Human cavernous hemangioma endothelial cells were isolated using existing methods (see Lou J., Ythier A., ​​Burger D. et al. Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I. J Neuroimmunology 1997, 77: 107-115.). Briefly, human cavernous hemangioma tissue was cut into 1-2 mm 3Then digested with 0.2% type I collagenase at 37°C for 15 minutes, and then added 0.1% trypsin / 0.1% EDTA solution to continue digestion for 5 minutes. Pass the digested tissue suspension through a 100-mesh copper mesh, collect the liquid, and centrifuge and wash twice with DMEM. The cells were mixed with 2ml of fetal bovine serum (Hyclone Company), and allowed to stand for 2 minutes, and human hemangioma endothelial cell culture medium (containing 20% ​​fetal bovine serum, 100 μg / ml ECGS, 40 U / ml heparin,...

Embodiment 2

[0046] Repeat embodiment 1, its difference is only: get 5 * 10 5 Individual hemangioma endothelial cells with 1 x 10 5 The human osteosarcoma cell line HOS cells are co-inoculated; the immunodeficiency experimental animals are SCID mice.

Embodiment 3

[0048] Repeat embodiment 1, its difference is only: get 1 * 10 6 The personal liver cancer cell line HepG2 was suspended in 1ml DMEM and crushed by ultrasonication. The ultrasonic condition was 10 seconds each time with an interval of 30 seconds. The supernatant was collected by centrifugation at 10000g for 10 minutes, and then 1×10 7 The human hemangioma endothelial cells are mixed with the supernatant and co-inoculated; the immunodeficiency experimental animals are SCID mice.

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Abstract

The present invention transplants hemangioma endothelial cell and tumor cell or tumor cell releasing product isolating cultured from human hemangioma structure, to immunologic deficiency mouse hypodermic, to obtain human hemangioma mouse animal model. The present invented technology method is portably in technical operation and with high model success ratio, the experimental evidence proving that said blood vessel endothelial cell in hemangioma model established is human hemangioma endothelial cell, and said model having the pathology property of human angiomatous intumescentia blood sinus and rebirth capillary vessel. Said hemangioma animal model can be used in system researching angiomatous pathogenesis screening human angiomatous therapeutic drug.

Description

technical field [0001] The invention relates to an animal model and a construction method thereof, in particular to a human hemangioma mouse animal model and a construction method thereof. Background technique [0002] Hemangioma is a common benign vascular malformation. According to its shape and pathological characteristics, it can be divided into three types: cavernous hemangioma, strawberry hemangioma and mangiform hemangioma. The incidence rate in infants and adolescents is as high as 1-3%. . Hemangiomas generally occur on the face of children, and can also occur in internal organs, and most of them increase with age. Those that occur on the face seriously affect the appearance, and those that occur on important organs may be damaged due to the rupture of the hemangioma. life threatening. [0003] The mechanism of hemangioma is still not fully understood. One theory holds that excessive angiogenesis is caused by uncontrolled regulation of local angiogenesis and produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
Inventor 张文健娄晋宁
Owner SUZHOU BOJUHUA BIOMEDICAL TECH CO LTD
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