H5N1 type poultry grippal virus fluorescent augmentation detection kit and detection method

An avian influenza virus, detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of low separation rate, high requirements, restrictions, etc., and achieve simple and convenient sampling, high sensitivity, and judgment. Convenient and accurate results
CN1904068AInactive Publication Date: 2007-01-31ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2007-01-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a kind of H5N1-type avian influenza virus fluorescence amplification detection kit and detection method for detecting avian influenza virus byusing said kit. Said kit includes the following several portions: H5N1-type avian influenza virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse transcriptase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR buffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect specific gene containing H5N1-type avian influenza virus.
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Description

(1) Technical field

[0001] The invention relates to a H5N1 type avian influenza virus fluorescence amplification detection kit and a detection method. (2) Background technology

[0002] Highly pathogenic avian influenza (HPAI) is an acute and severe respiratory infectious disease caused by H5N1 avian influenza virus. Its morbidity and mortality are extremely high. It is one of the main severe infectious diseases that endanger poultry. class of infectious diseases. In recent years, studies have found that the highly pathogenic avian influenza virus H5N1 can break through the racial barrier, infect humans and cause disease or even death. Since 2003, the number of deaths due to the infection of avian influenza H5N1 virus in Asia and my country has reached 64 people, making it one of the vicious infectious diseases that seriously endanger human health. Rapid mutation has posed a great threat to human health. Therefore, the establishment of rapid and accurate H5 avian influenza...

Claims

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