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Method of assaying cholesterol of high-density lipoprotein

A high-density lipoprotein and cholesterol technology, which is applied in the field of determination of cholesterol in high-density lipoprotein, can solve problems such as inaccurate cholesterol measurement data and incomplete elimination of cholesterol

Inactive Publication Date: 2007-02-21
KYOWA MEDEX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in these methods of measuring HDL cholesterol without aggregating lipoproteins other than HDL, cholesterol in lipoproteins other than HDL may not be completely eliminated, and cholesterol in lipoproteins other than HDL may be affected. The problem of inaccurate measurement data caused by non-specific reactions

Method used

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  • Method of assaying cholesterol of high-density lipoprotein
  • Method of assaying cholesterol of high-density lipoprotein
  • Method of assaying cholesterol of high-density lipoprotein

Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[1191] Reference Example 1 Preparation of chemically modified LPL311 (chemically modified LPL311)

[1192] Add LPL311 to HEPES buffer (pH8.5 0.15mol / L) to make the concentration 33g / L, cool to 5°C, add Sanbride (Sanbride) VFM-4101 (manufactured by NOF Corporation) to make the concentration 330g / L, and then reacted for 3 hours. The obtained modified enzyme solution was directly used as chemically modified LPL311 without purification.

Embodiment 1

[1194] A kit for HDL cholesterol measurement consisting of the following first reagent (reagent A) and second reagent (reagent a) was prepared.

[1195] First Reagent (Reagent A)

[1196] HEPES(pH7.5) 10mmol / L

[1197] EMSE0.3g / L

[1198] The second reagent (reagent a 1 )

[1199] HEPES(pH7.0) 10mmol / L

[1200] 4-Aminoantipyrine 0.3g / L

[1201] Catalase 20kU / L

[1202] Chemically modified LPL311 0.2kU / L

[1203] COO321 5.0kU / L

[1204] BLAUNON SAP3004 0.1g / L

Embodiment 2

[1206] The following first reagent (reagent A) and second reagent (reagent a) were prepared 2 ) composed of HDL cholesterol assay kit.

[1207] First Reagent (Reagent A)

[1208] HEPES(pH7.5) 10mmol / L

[1209] EMSE0.3g / L

[1210] The second reagent (reagent a 2 )

[1211] HEPES(pH7.0) 10mmol / L

[1212]4-Aminoantipyrine 0.3g / L

[1213] Catalase 20kU / L

[1214] Chemically modified LPL311 0.2kU / L

[1215] COO321 5.0kU / L

[1216] BLAUNON SAP3010 0.5g / L

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Abstract

A method of assaying cholesterol of high-density lipoprotein in an analyte, comprising reacting an analyte with (i) cholesterol ester hydrolase and cholesterol oxidase, or (ii) cholesterol ester hydrolase, oxidized coenzyme and cholesterol dehydrogenase, in an aqueous medium containing at least one substance selected from the group consisting of an alkylamine polyoxyethylene polyoxypropylene condensate, a polyoxyethylenealkylamine sulfate, a polyoxyethylenebenzyl-alkyl quaternary ammonium salt, a polyoxyethylene acid amide, an alkylamine oxide and an alkylpropylenediamine derivative to thereby produce hydrogen peroxide or reduced coenzyme, and measuring the produced hydrogen peroxide or reduced coenzyme.

Description

technical field [0001] The invention relates to a method for measuring cholesterol in high-density lipoprotein in a sample, a reagent for the test and a kit for the test. Background technique [0002] Lipoproteins in living organisms are divided into high-density lipoproteins (hereinafter abbreviated as HDL), low-density lipoproteins (hereinafter abbreviated as LDL), very low-density lipoproteins (hereinafter abbreviated as VLDL), and chylomicrons (hereinafter abbreviated as CM) according to specific gravity. ), each plays a very different role in the body due to the different types of apolipoproteins, and the composition of lipids is also varied. It has been known that HDL accepts cholesterol from various tissues including arterial blood vessel walls, and thus is related to the removal of cholesterol accumulated in cells, and is the key to preventing various arterial diseases mainly caused by coronary atherosclerosis. Its level in the blood serves as a useful indicator for...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/28C12Q1/32C12Q1/44G01N33/92
CPCG01N33/92C12Q1/44
Inventor 片山有基藤中真由美
Owner KYOWA MEDEX CO LTD
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